Anticorps Polyclonal de lapin anti-INSC

INSC Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 20973-1-AP

Synonymes

INSC, Protein inscuteable homolog

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Galerie de données de validation

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  WB analysis of mouse liver using 20973-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20973-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20973-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20973-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20973-1-AP (INSC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 20973-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20973-1-AP (INSC antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 20973-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20973-1-AP (INSC antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 20973-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20973-1-AP (INSC antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 20973-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20973-1-AP (INSC antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 20973-1-AP

  Immunofluorescent analysis of HeLa cells using 20973-1-AP (INSC antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• ChIP: See 1 publications below

Informations sur le produit

20973-1-AP cible INSC dans les applications de WB, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: inscuteable homolog (Drosophila)
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: NM_001031853
• Symbole du gène: INSC
• Identification du gène (NCBI): 387755
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

INSC may function as an adapter linking the Par3 complex to the GPSM1/GPSM2 complex. It is involved in spindle orientation during mitosis it may regulate cell proliferation and differentiation in the developing nervous system. INSC may play a role in the asymmetric division of fibroblasts and participate in the process of stratification of the squamous epithelium. The antibody can recognize isoform1, 2,4,5 of INSC.

Publications

Species	Application	Title
	ChIP	Cell Rep; The transcription factor ZEB1 regulates stem cell self-renewal and cell fate in the adult hippocampus.; Authors - Bhavana Gupta