Anticorps Polyclonal de lapin anti-INSIG1

INSIG1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(10)

Avis (1)

N° de cat : 55282-1-AP

Synonymes

CL 6, INSIG 1, INSIG-1, insulin induced gene 1, Insulin induced gene 1 protein

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Galerie de données de validation

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  WB analysis using 55282-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55282-1-AP (INSIG1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 55282-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55282-1-AP (INSIG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 55282-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55282-1-AP (INSIG1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 55282-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using INSIG1 antibody (55282-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de rat
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

55282-1-AP cible INSIG1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: insulin induced gene 1
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: NM_005542
• Symbole du gène: INSIG1
• Identification du gène (NCBI): 3638
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Insulin-induced genes (INSIGs), which consist of two isoforms (INSIG1 and INSIG2), are representative endoplasmic reticulum (ER)-resident oxysterol-binding proteins (OSBPs). INSIG1 is part of negative regulatory feedback, acting as a brake on SREBP transcriptional function (PMID: 23919961). INSIG1 also has a secondary function mediating sterolaccelerated proteolytic degradation of HMG CoA reductase, thus impacting the mevalonate pathway. Suppression of INSIG1 function promotes hepatic lipid remodelling and restrains NASH (non-alcoholic steatohepatitis) progression(PMID: 33722690).

Publications

Species	Application	Title
		Cell Death Differ; Ubiquitination of NLRP3 by gp78/Insig-1 restrains NLRP3 inflammasome activation.; Authors - Ting Xu
mouse	WB	Front Pharmacol; Acacetin protects against depression-associated dry eye disease by regulating ubiquitination of NLRP3 through gp78 signal; Authors - Mingxia Xie
chicken	WB,IF	Poult Sci; Functional and miRNA regulatory characteristics of INSIG genes highlight the key role of lipid synthesis in the liver of chicken (Gallus gallus); Authors - Yaxin Yue
mouse,human	WB,IF	FASEB J; Dickkopf-1 promotes vascular smooth muscle cell foam cell formation and atherosclerosis development through CYP4A11/SREBP2/ABCA1; Authors - Xiao Li
mouse,human	WB	Metabolism; miR-32-5p induces hepatic steatosis and hyperlipidemia by triggering de novo lipogenesis; Authors - Ya-Di Wang
human	WB	Redox Biol; Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis; Authors - Xue Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zach (Verified Customer) (09-12-2025)

Did not detect endogenous protein but detected transgenically overexpressed ones.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: liver

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