Anticorps Polyclonal de lapin anti-INTS8

INTS8 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 18802-1-AP

Synonymes

Protein kaonashi-1, Protein kaonashi 1, Integrator complex subunit 8, Int8, C8orf52

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Galerie de données de validation

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  WB analysis of MCF-7 using 18802-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18802-1-AP (INTS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18802-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18802-1-AP (INTS8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18802-1-AP

  IP result of anti-INTS8 (IP:18802-1-AP, 4ug; Detection:18802-1-AP 1:500) with HeLa cells lysate 4000ug.

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  IF Staining of HeLa using 18802-1-AP

  Immunofluorescent analysis of Hela cells, using INTS8 antibody 18802-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules MCF7, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

18802-1-AP cible INTS8 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: integrator complex subunit 8
• Masse moléculaire calculée: 111 kDa
• Poids moléculaire observé: 95-111 kDa
• Numéro d’acquisition GenBank: BC136754
• Symbole du gène: INTS8
• Identification du gène (NCBI): 55656
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Integrator complex subunit 8 (INTS8) is one of the major components of RNA polymerase II and has been demonstrated to be involved in the cleavage of small nuclear RNAs and transcriptional processes (PMID: 34880328). INTS8 was robustly increased in neurodevelopmental diseases and numerous tumors. High INTS8 expression has a tight correlation with poor prognosis in multi-cancers (PMID: 30881114). Western blot analysis detected INTS8 at an apparent molecular mass of 111 kDa.

Publications

Species	Application	Title
mouse	WB	Science; Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.; Authors - Hai Zheng
human	WB	J Exp Clin Cancer Res; Microarray-based identification of genes associated with cancer progression and prognosis in hepatocellular carcinoma.; Authors - Fuqiang Yin
human	IHC	Cancer Manag Res; INTS8 accelerates the epithelial-to-mesenchymal transition in hepatocellular carcinoma by upregulating the TGF-β signaling pathway.; Authors - Hui Tong
human	WB	Cell Rep; A homozygous variant in INTS11 links mitosis and neurogenesis defects to a severe neurodevelopmental disorder; Authors - Hanzhe Kuang
human	WB	Elife; Human promoter directionality is determined by transcriptional initiation and the opposing activities of INTS11 and CDK9; Authors - Joshua D Eaton

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