Anticorps Polyclonal de lapin anti-IRF1

• Phare
• Validé par KD/KO

IRF1 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

27

N° de cat : 11335-1-AP

Synonymes

IRF 1, IRF1, MAR

---

Galerie de données de validation

• 

  WB analysis of HL-60 using 11335-1-AP

  WB result of IRF1 antibody (11335-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRF1 transfected HL-60 cells.

• 

  WB analysis using 11335-1-AP

  Western blot analysis of HeLa cells, untreated or treated with Human IFN-γ (hIFN-γ) （HZ-1301） (100 ng/ml, 48 hr), using 11335-1-AP (IRF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11335-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11335-1-AP (IRF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 11335-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11335-1-AP (IRF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11335-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11335-1-AP (IRF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 11335-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11335-1-AP (IRF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 11335-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11335-1-AP (IRF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 11335-1-AP

  IP result of anti-IRF1 (IP:11335-1-AP, 4ug; Detection:11335-1-AP 1:300) with HL-60 cells lysate 4400 ug.

• 

  IHC staining of human breast cancer using 11335-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11335-1-AP (IRF1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 11335-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11335-1-AP (IRF1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  FC experiment of Jurkat using 11335-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human IRF1 (11335-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

---

Applications testées

• Résultats positifs en WB: cellules HeLa traitées à l'IFN gamma, cellules HL-60, cellules Jurkat, cellules Raji
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 24 publications below
• IHC: See 5 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

11335-1-AP cible IRF1 dans les applications de WB, IHC, IF, FC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IRF1 Protéine recombinante Ag1879
• Nom complet: IRF 1
• Masse moléculaire calculée: 325 aa, 37 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC009483
• Symbole du gène: IRF1
• Identification du gène (NCBI): 3659
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IRF1, also named as IFN regulatory factor 1, is a 325 amino acid protein, which belongs to the IRF family. IRF1 as a transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. The calculated molecular weight of IRF1 is 37 kDa, but the modified IRF1 protein is about 45-50 kDa.

Publications

Species	Application	Title
human	WB	J Immunother Cancer; Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis.; Authors - Weinan Guo
human	WB,IHC,IF	Free Radic Biol Med; IRF1/ZNF350/GPX4-mediated ferroptosis of renal tubular epithelial cells promote chronic renal allograft interstitial fibrosis; Authors - Yao Zhang ; KD Validated
human	WB	Front Immunol; Toxoplasma Effector TgIST Targets Host IDO1 to Antagonize the IFN-γ-Induced Anti-parasitic Response in Human Cells.; Authors - Hironori Bando
human	WB	Cancer Commun (Lond); MAGE-C3 promotes cancer metastasis by inducing epithelial-mesenchymal transition and immunosuppression in esophageal squamous cell carcinoma.; Authors - Qingnan Wu
mouse	IF	Int J Mol Sci; Comprehensive Analysis of PANoptosis-Related Gene Signature of Ulcerative Colitis; Authors - Jun-Meng Wang
rat	WB	Inflammation; Chlorogenic Acid Alleviates Hepatic Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Mitochondria-Mediated Apoptosis In Vivo and In Vitro; Authors - Kai Li