Anticorps Polyclonal de lapin anti-IRF2

• Phare
• Validé par KD/KO

IRF2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 12525-1-AP

Synonymes

IRF 2, IRF2

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Galerie de données de validation

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  WB analysis using 12525-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12525-1-AP (IRF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 12525-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12525-1-AP (IRF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12525-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12525-1-AP (IRF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 12525-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12525-1-AP (IRF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12525-1-AP

  IP Result of anti-IRF2 (IP:12525-1-AP, 4ug; Detection:12525-1-AP 1:500) with Jurkat cells lysate 2800ug.

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  IHC staining of human colon cancer using 12525-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12525-1-AP (IRF2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 12525-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12525-1-AP (IRF2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of Jurkat using 12525-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using IRF2 antibody (12525-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COLO 320, cellules Jurkat, tissu de côlon de souris
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Informations sur le produit

12525-1-AP cible IRF2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IRF2 Protéine recombinante Ag3220
• Nom complet: IRF 2
• Masse moléculaire calculée: 349 aa, 39 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC015803
• Symbole du gène: IRF2
• Identification du gène (NCBI): 3660
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IRF2, also known as IFN regulatory factor 2, which belongs to the IRF family. IRF2 specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the IFN consensus sequence (ICS)) and represses those genes. IRF2 also acts as an activator for several genes including H4 and IL7. IRF2 constitutively binds to the ISRE promoter to activate IL7. IRF2 is involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. The calculated molecular weight of IRF2 is 39 kDa, but post-modification of IRF2 is about 50 kDa (PMID: 17698501).

Publications

Species	Application	Title
chicken	WB	Int J Mol Sci; Analysis of miRNA Expression Profiling of RIP2 Knockdown in Chicken HD11 Cells When Infected with Avian Pathogenic E. coli (APEC).; Authors - Hongyan Sun
human	WB	Virol J; IRF2 inhibits ZIKV replication by promoting FAM111A expression to enhance the host restriction effect of RFC3.; Authors - Kai Ren ; KD Validated
mouse	WB	J Immunol Res; Propofol Ameliorates Microglia Activation by Targeting MicroRNA-221/222-IRF2 Axis.; Authors - Xi Xiao ; KD Validated
human	WB,IHC	J Dermatol Sci; PMID: 31138473; Authors - Xiangzhi Li ; KD Validated
human	WB	Transl Oncol; IRF2-ferroptosis related gene is associated with prognosis and EMT in gliomas; Authors - Shiao Tong ; KD Validated
human	WB	Pathol Res Pract; Long noncoding RNA SENCR facilitates the progression of acute myeloid leukemia through the miR-4731-5p/IRF2 pathway; Authors - Changhao Han