Anticorps Polyclonal de lapin anti-IRF2BP2

• Phare
• Validé par KD/KO

IRF2BP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 18847-1-AP

Synonymes

IRF2BP 2, IRF 2BP2, Interferon regulatory factor 2-binding protein 2

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Galerie de données de validation

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  WB analysis of HeLa using 18847-1-AP

  WB result of IRF2BP2 antibody (18847-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRF2BP2 transfected HeLa cells.

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  WB analysis using 18847-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18847-1-AP (IRF2BP2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 18847-1-AP

  IP result of anti-IRF2BP2 (IP:18847-1-AP, 4ug; Detection:18847-1-AP 1:6000) with K-562 cells lysate 1820 ug.

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  IHC staining of human lung squamous cell carcinoma using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 18847-1-AP (IRF2BP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18847-1-AP (IRF2BP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18847-1-AP (IRF2BP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18847-1-AP (IRF2BP2 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 18847-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IRF2BP2 antibody (18847-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules K-562
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: human lung squamous cell carcinoma tissue, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

18847-1-AP cible IRF2BP2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: interferon regulatory factor 2 binding protein 2
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: NM_182972
• Symbole du gène: IRF2BP2
• Identification du gène (NCBI): 359948
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IRF2BP2 belongs to the IRF2BP family. IRF2BP2 acts as a transcriptional repressor and as a transcriptional corepressor in a IRF2-dependent manner. This repression is not mediated at least in part by histone deacetylase activities.

Publications

Species	Application	Title
human	WB	Cancer Discov; Unleashing Cell-Intrinsic Inflammation as a Strategy to Kill AML Blasts.; Authors - Jana M Ellegast ; KO Validated
		Hepatology; The novel tumor suppressor IRF2BP2 regulates Hippo pathway in liver cancer via a feedback loop.; Authors - Xue Feng
human	WB,IF	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
human	WB,IP	Cell Death Dis; SPOP point mutations regulate substrate preference and affect its function; Authors - Yanran Deng
human	ChIP	EMBO Rep; Transient deSUMOylation of IRF2BP proteins controls early transcription in EGFR signaling.; Authors - Sina V Barysch
mouse	WB,IHC	Hypertension; Control of Pathological Cardiac Hypertrophy by Transcriptional Corepressor IRF2BP2 (Interferon Regulatory Factor-2 Binding Protein 2).; Authors - Jing Fang ; KO Validated

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