Anticorps Polyclonal de lapin anti-IRF2BP2

• Phare
• Validé par KD/KO

IRF2BP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 18847-1-AP

Synonymes

IRF 2 binding protein 2, IRF 2BP2, IRF2BP2

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Galerie de données de validation

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  WB analysis of Jurkat using 18847-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18847-1-AP (IRF2BP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18847-1-AP

  WB result of IRF2BP2 antibody (18847-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRF2BP2 transfected HeLa cells.

• 

  WB analysis of HepG2 using 18847-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18847-1-AP (IRF2BP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 18847-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18847-1-AP (IRF2BP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 18847-1-AP

  IP Result of anti-IRF2BP2 (IP:18847-1-AP, 3ug; Detection:18847-1-AP 1:500) with K-562 cells lysate 2400ug.

• 

  IHC staining of human lung cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18847-1-AP (IRF2BP2 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18847-1-AP (IRF2BP2 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18847-1-AP (IRF2BP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18847-1-AP (IRF2BP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18847-1-AP (IRF2BP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 18847-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18847-1-AP (IRF2BP2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 18847-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 18847-1-AP (IRF2BP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules HepG2, cellules K-562
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

18847-1-AP cible IRF2BP2 dans les applications de WB, IP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: interferon regulatory factor 2 binding protein 2
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: NM_182972
• Symbole du gène: IRF2BP2
• Identification du gène (NCBI): 359948
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IRF2BP2 belongs to the IRF2BP family. IRF2BP2 acts as a transcriptional repressor and as a transcriptional corepressor in a IRF2-dependent manner. This repression is not mediated at least in part by histone deacetylase activities.

Publications

Species	Application	Title
human	WB	Cancer Discov; Unleashing Cell-Intrinsic Inflammation as a Strategy to Kill AML Blasts.; Authors - Jana M Ellegast ; KO Validated
		Hepatology; The novel tumor suppressor IRF2BP2 regulates Hippo pathway in liver cancer via a feedback loop.; Authors - Xue Feng
human	WB,IF	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
human	ChIP	EMBO Rep; Transient deSUMOylation of IRF2BP proteins controls early transcription in EGFR signaling.; Authors - Sina V Barysch
mouse	WB,IHC	Hypertension; Control of Pathological Cardiac Hypertrophy by Transcriptional Corepressor IRF2BP2 (Interferon Regulatory Factor-2 Binding Protein 2).; Authors - Jing Fang ; KO Validated
human	WB	PLoS Genet; Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.; Authors - Emily M Schleicher

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