Anticorps Polyclonal de lapin anti-IRF5

IRF5 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

35

N° de cat : 10547-1-AP

Synonymes

interferon regulatory factor 5, IRF 5, IRF5, SLEB10

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Galerie de données de validation

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  WB analysis using 10547-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 10547-1-AP (IRF5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10547-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10547-1-AP (IRF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 10547-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10547-1-AP (IRF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10547-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10547-1-AP (IRF5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10547-1-AP

  IP Result of anti-IRF5 (IP:10547-1-AP, 4ug; Detection:10547-1-AP 1:300) with HeLa cells lysate 1600ug.

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  IP experiment of HeLa using 10547-1-AP

  IP Result of anti-IRF5 (IP:10547-1-AP, 4ug; Detection:10547-1-AP 1:300) with HeLa cells lysate 1600ug.

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  IHC staining of human prostate cancer using 10547-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10547-1-AP (IRF5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10547-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10547-1-AP (IRF5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10547-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10547-1-AP (IRF5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10547-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10547-1-AP (IRF5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10547-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10547-1-AP (IRF5 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 10547-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IRF5 antibody (10547-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 10547-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IRF5 antibody (10547-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 10547-1-AP

  Immunofluorescent analysis of HepG2 cells using 10547-1-AP (IRF5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 10547-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug IRF5 antibody (10547-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules Jurkat, cellules SH-SY5Y
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 21 publications below
• IHC: See 9 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

10547-1-AP cible IRF5 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IRF5 Protéine recombinante Ag0841
• Nom complet: interferon regulatory factor 5
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC004201
• Symbole du gène: IRF5
• Identification du gène (NCBI): 3663
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. It is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10). Alternative splice variant encoding different isoforms exist. Catalog#10547-1-AP is a rabbit polyclonal antibody raised against the C-terminal of human IRF5.

Publications

Species	Application	Title
human	IF	Br J Sports Med; Chronic inflammation is a feature of Achilles tendinopathy and rupture.; Authors - Stephanie Georgina Dakin
mouse	WB	J Immunother Cancer; Ccl3 enhances docetaxel chemosensitivity in breast cancer by triggering proinflammatory macrophage polarization.; Authors - Dandan Sheng
rat	WB	Am J Transplant; Characterization of Transfusion-Elicited Acute Antibody-Mediated Rejection in a Rat Model of Kidney Transplantation.; Authors - G Huang
mouse	WB	J Control Release; PEGylation of interleukin-10 improves the pharmacokinetic profile and enhances the antifibrotic effectivity in CCl?-induced fibrogenesis in mice.; Authors - Mattos Adriana A
mouse	IHC	Br J Pharmacol; Ibuprofen and diclofenac treatments reduce proliferation of pancreatic acinar cells upon inflammatory injury and mitogenic stimulation.; Authors - Marta Bombardo
mouse	WB,IF	Biochem Pharmacol; Quercetin ameliorates kidney injury and fibrosis by modulating M1/M2 macrophage polarization.; Authors - Hong Lu

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