Anticorps Recombinant de lapin anti-IRF7

IRF7 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242613C2

N° de cat : 85072-5-RR

Synonymes

242613C2, IRF 7, IRF 7H, IRF-7, IRF7A

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Galerie de données de validation

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  WB analysis using 85072-5-RR

  IFN alpha treated and untreated Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 85072-5-RR (IRF7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of Jurkat using 85072-5-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug IRF7 Recombinant antibody (85072-5-RR, Clone:242613C2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 85072-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85072-5-RR against Human IRF7 were performed. The affinity constant is 13.1 pM.

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Applications testées

• Résultats positifs en WB: IFN alpha treated Jurkat cells,
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85072-5-RR cible IRF7 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IRF7 Protéine recombinante Ag18059
• Nom complet: IRF 7
• Masse moléculaire calculée: 516 aa, 56 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC136555
• Symbole du gène: IRF7
• Identification du gène (NCBI): 3665
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IRF-7 (IFN regulatory factor 7) is a member of the IFN regulatory transcription factor (IRF) family. IRF-7 has been shown to play a role in the transcriptional activation of virus-inducible cellular proteins, including IFN beta chain proteins. Inducible expression of IRF-7 is largely restricted to lymphoid tissue. Four transcript variants encoding distinct isoforms (A,B,C and D) have been identified for this (PMID:9786932).The active IRF7A exists as a dimer form ~80 kDa(PMID:11073981). The MW 67-70 kDa has been reported in some papers (PMID:9786932; 22951831).Various posttranslational modifications of IRF7 including phosphorylation, ubiquitination, sumoylation and acetylation are identified (PMID:22951831).

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