Anticorps Polyclonal de lapin anti-Integrin Alpha 3

• Phare
• Validé par KD/KO

Integrin Alpha 3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 21992-1-AP

Synonymes

CD49C, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a

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Galerie de données de validation

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  WB analysis of A549 using 21992-1-AP

  WB result of Integrin Alpha 3 antibody (21992-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 3 transfected A549 cells.

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  WB analysis using 21992-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21992-1-AP (Integrin Alpha 3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 21992-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21992-1-AP (ITGA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 21992-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21992-1-AP (ITGA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 21992-1-AP

  IP Result of anti-Integrin alpha-3 (IP:21992-1-AP, 4ug; Detection:21992-1-AP 1:500) with PC-3 cells lysate 1200ug.

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  IHC staining of mouse colon using 21992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21992-1-AP (Integrin Alpha 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 21992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21992-1-AP (Integrin Alpha 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 21992-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Integrin Alpha 3 antibody (21992-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of PC-3 using 21992-1-AP

  1X10^6 PC-3 cells were stained with 0.2ug Integrin alpha-3 antibody (21992-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules PC-3, tissu cardiaque humain, tissu testiculaire humain
• Résultats positifs en IP: cellules PC-3
• Résultats positifs en IHC: tissu de côlon de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549,
• Résultats positifs en cytométrie: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

21992-1-AP cible Integrin Alpha 3 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Integrin Alpha 3 Protéine recombinante Ag17293
• Nom complet: integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
• Masse moléculaire calculée: 1066 aa, 119 kDa
• Poids moléculaire observé: 130-160 kDa
• Numéro d’acquisition GenBank: BC150190
• Symbole du gène: ITGA3
• Identification du gène (NCBI): 3675
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.

Publications

Species	Application	Title
human	WB	Front Oncol; High Expression of Integrin α3 Predicts Poor Prognosis and Promotes Tumor Metastasis and Angiogenesis by Activating the c-Src/Extracellular Signal-Regulated Protein Kinase/Focal Adhesion Kinase Signaling Pathway in Cervical Cancer.; Authors - Qiqiao Du
human,mouse	WB,IF	Nat Commun; Integrin α3β1 promotes vessel formation of glioblastoma-associated endothelial cells through calcium-mediated macropinocytosis and lysosomal exocytosis.; Authors - Eunnyung Bae

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