Anticorps Monoclonal anti-Integrin Alpha 3

• Phare
• Validé par KD/KO

Integrin Alpha 3 Monoclonal Antibody for FC, IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

15

CloneNo.

3F9G4

Avis (2)

N° de cat : 66070-1-Ig

Synonymes

CD49C, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a

---

Galerie de données de validation

• 

  WB analysis of A549 using 66070-1-Ig

  WB result of ITGA3 antibody (66070-1-Ig, 1:6000) with si-control and si-ITGA3 transfected A549 cells.

• 

  WB analysis of PC-3 using 66070-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (ITGA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66070-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 66070-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66070-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66070-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 66070-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66070-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of PC-3 using 66070-1-Ig

  1X10^6 PC-3 cells were stained with 0.2 ug Anti-Human Integrin Alpha 3 (66070-1-Ig, Clone: 3F9G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug Mouse IgG2a Isotype Control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH.

• 

  FC experiment of A549 using 66070-1-Ig

  1X10^6 A549 cells were stained with 0.2ug Integrin alpha-3 antibody (66070-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

---

Applications testées

• Résultats positifs en WB: cellules PC-3, cellules A431, cellules A549, cellules HepG2, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules PC-3, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• FC: See 2 publications below

Informations sur le produit

66070-1-Ig cible Integrin Alpha 3 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: Humain, Lapin, porc, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Integrin Alpha 3 Protéine recombinante Ag17293
• Nom complet: integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
• Masse moléculaire calculée: 1066 aa, 119 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC150190
• Symbole du gène: ITGA3
• Identification du gène (NCBI): 3675
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.

Publications

Species	Application	Title
human	WB, IF	Mol Cell Proteomics; Hypoxia-induced changes to integrin alpha 3 glycosylation facilitate invasion in epidermoid carcinoma cell line A431.; Authors - Yan Ren
mouse	WB	CNS Neurosci Ther; Integrin α3 is required for high-frequency repetitive transcranial magnetic stimulation-induced glutamatergic synaptic transmission in mice with ischemia; Authors - Li Liu
human	IHC	Thyroid; Clinical and Molecular Characterizations of Papillary Thyroid Cancer in Children and Young Adults: a Multicenter Retrospective Study.; Authors - Kai Guo
human	IHC	Front Mol Biosci; Golgi Phosphoprotein 3 Promotes Colon Cancer Cell Metastasis Through STAT3 and Integrin α3 Pathways.; Authors - Anpei Huang
human	FC	Cell Adh Migr; Tissue factor potentiates adherence of breast cancer cells to human umbilical vein endothelial cells under static and flow conditions.; Authors - Yanling Jin
human	IF	Mol Hum Reprod; Angiopoietin 2 stimulates trophoblast invasion via a mechanism associated with JNK signaling.; Authors - Huomei Hou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other

Produits associés

Integrin Alpha 3 Polyclonal antibody

21992-1-AP

Integrin Beta 1 Monoclonal antibody

66315-1-Ig

Integrin Beta 1 Polyclonal antibody

12594-1-AP

Integrin Alpha 6 Polyclonal antibody

27189-1-AP

CD41/Integrin Alpha 2B Polyclonal antibody

24552-1-AP

Integrin Alpha-5 Polyclonal antibody

10569-1-AP