Anticorps Polyclonal de lapin anti-Integrin Alpha 5

• Phare
• Validé par KD/KO

Integrin Alpha 5 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 27224-1-AP

Synonymes

CD49e, FNRA, Integrin alpha 5, Integrin alpha F, ITGA5, VLA 5, VLA5A

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Galerie de données de validation

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  WB analysis using 27224-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27224-1-AP

  WB result of Integrin alpha-5 antibody (27224-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin alpha-5 transfected HeLa cells.

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  WB analysis of human placenta using 27224-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 27224-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27224-1-AP (ITGA5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27224-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 27224-1-AP

  IP result of anti-Integrin alpha-5 (IP:27224-1-AP, 4ug; Detection:27224-1-AP 1:1000) with human placenta tissue lysate 2600 ug.

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  IP experiment of human placenta using 27224-1-AP

  IP result of anti-Integrin alpha-5 (IP:27224-1-AP, 4ug; Detection:27224-1-AP 1:1000) with human placenta tissue lysate 4000 ug.

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  IHC staining of human placenta using 27224-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 27224-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules U2OS, tissu placentaire humain
• Résultats positifs en IP: tissu placentaire humain,
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

27224-1-AP cible Integrin Alpha 5 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Integrin Alpha 5 Protéine recombinante Ag26098
• Nom complet: integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• Masse moléculaire calculée: 115 kDa
• Poids moléculaire observé: 135-150 kDa
• Numéro d’acquisition GenBank: NM_002205
• Symbole du gène: ITGA5
• Identification du gène (NCBI): 3678
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (01-25-2019)

By WB, it labels a band at the expected molecular weight, but this band is very likely not the real protein, because it is increased during adipocyte differentiation, which should be decreased during the process according to literature.By IF, PFA fixation, it labels the protein in the nucleus. But according to literature, it is localised in the cell surface and the Gogli.

• Applications: Western Blot, Immunofluorescence,