Anticorps Polyclonal de lapin anti-CD11B/Integrin Alpha M

• Phare
• Validé par KD/KO

CD11B/Integrin Alpha M Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

26

N° de cat : 21851-1-AP

Synonymes

CD11B, CD11B/Integrin Alpha M, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6

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Galerie de données de validation

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  WB analysis using 21851-1-AP

  TF-1 cells were subjected to SDS PAGE followed by western blot with 21851-1-AP (CD11B/Integrin Alpha M antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 21851-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21851-1-AP (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 21851-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21851-1-AP (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21851-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21851-1-AP (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21851-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21851-1-AP (CD11B/Integrin alpha M antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Insulinoma using 21851-1-AP

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 21851-1-AP (CD11B/Integrin alpha M antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Insulinoma using 21851-1-AP

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 21851-1-AP (CD11B/Integrin alpha M antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of THP-1 using 21851-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD11B/Integrin Alpha M antibody (21851-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules TF-1,
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu d'insulinome; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 10 publications below
• IF: See 13 publications below
• FC: See 1 publications below

Informations sur le produit

21851-1-AP cible CD11B/Integrin Alpha M dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD11B/Integrin Alpha M Protéine recombinante Ag16327
• Nom complet: integrin, alpha M (complement component 3 receptor 3 subunit)
• Masse moléculaire calculée: 1152 aa, 127 kDa
• Poids moléculaire observé: 170 kDa
• Numéro d’acquisition GenBank: BC096346
• Symbole du gène: ITGAM
• Identification du gène (NCBI): 3684
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Publications

Species	Application	Title
human	IHC,WB	Aging Cell; Advanced maternal age causes premature placental senescence and malformation via dysregulated α-Klotho expression in trophoblasts.; Authors - Zhi Chen
	FC	Biomaterials; Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia.; Authors - Haoran Wang
	IF	ACS Appl Mater Interfaces; Catalysis-Mediated Male Contraception through Black Phosphorus Nanosheets; Authors - Lirong Yi
	IF	Int J Biol Macromol; Repairing gastric ulcer with hyaluronic acid/extracellular matrix composite through promoting M2-type polarization of macrophages; Authors - Renhao Ni
	IF	Biomater Sci; A hybrid hydrogel composed of chitin and β-glucan for the effective management of wound healing and scarring; Authors - Ximeng Kang
human	WB	Int J Mol Sci; Bidirectional Tumor-Promoting Activities of Macrophage Ezrin.; Authors - Krishnendu Khan