Anticorps Polyclonal de lapin anti-Integrin Beta 4

• Phare
• Validé par KD/KO

Integrin Beta 4 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 21738-1-AP

Synonymes

CD104, GP150, Integrin beta 4, integrin, beta 4, ITGB4

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Galerie de données de validation

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  WB analysis using 21738-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21738-1-AP (Integrin beta-4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 21738-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21738-1-AP (ITGB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 21738-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21738-1-AP (ITGB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21738-1-AP

  IP Result of anti-Integrin beta-4 (IP:21738-1-AP, 4ug; Detection:21738-1-AP 1:500) with HeLa cells lysate 2400ug.

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  IHC staining of mouse colon using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21738-1-AP (Integrin Beta 4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21738-1-AP (Integrin Beta 4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21738-1-AP (Integrin beta-4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21738-1-AP (Integrin beta-4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21738-1-AP (Integrin Beta 4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21738-1-AP (Integrin Beta 4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of A431 using 21738-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Integrin Beta 4 antibody (21738-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 21738-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Integrin beta-4 antibody (21738-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules A431, cellules A549, cellules HeLa, cellules HT-29, cellules MCF-7
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de côlon de souris, tissu de cancer du pancréas humain, tissu de cancer du sein humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

21738-1-AP cible Integrin Beta 4 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Integrin Beta 4 Protéine recombinante Ag16450
• Nom complet: integrin, beta 4
• Masse moléculaire calculée: 1752 aa, 195 kDa
• Poids moléculaire observé: 200-240 kDa
• Numéro d’acquisition GenBank: BC126411
• Symbole du gène: ITGB4
• Identification du gène (NCBI): 3691
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.

Publications

Species	Application	Title
human,mouse	WB,IHC,CoIP	Oncogene; Programmed death ligand 1 promotes lymph node metastasis and glucose metabolism in cervical cancer by activating integrin β4/SNAI1/SIRT3 signaling pathway.; Authors - Shaojia Wang ; KD Validated
rat	WB	Phytomedicine; Ganoderma lucidum polysaccharide inhibits HSC activation and liver fibrosis via targeting inflammation, apoptosis, cell cycle, and ECM-receptor interaction mediated by TGF-β/Smad signaling; Authors - Chaojie Chen
human	WB,IHC,IF	Sci Rep; Integrin β4 promotes cell invasion and epithelial-mesenchymal transition through the modulation of Slug expression in hepatocellular carcinoma.; Authors - Xiao-Long Li
human	WB	Front Oncol; p63 Directs Subtype-Specific Gene Expression in HPV+ Head and Neck Squamous Cell Carcinoma.; Authors - Alexandra Ruth Glathar
human	WB,IHC	Cell Commun Signal; METTL14-mediated N6-methyladenosine modification of ITGB4 mRNA inhibits metastasis of clear cell renal cell carcinoma.; Authors - Zhuonan Liu ; KD Validated
human	IF	Int J Oncol; FAM83A exerts tumor‑suppressive roles in cervical cancer by regulating integrins.; Authors - Junfen Xu

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