Anticorps Polyclonal de lapin anti-ITIH3

ITIH3 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 21247-1-AP

Synonymes

Inter-alpha-inhibitor heavy chain 3, Inter-alpha-trypsin inhibitor heavy chain H3, ITI HC3, ITI heavy chain H3, ITI-HC3

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Galerie de données de validation

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  WB analysis of human plasma using 21247-1-AP

  human serum were subjected to SDS PAGE followed by western blot with 21247-1-AP (ITIH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 21247-1-AP

  IP result of anti-ITIH3 (IP:21247-1-AP, 4ug; Detection:21247-1-AP 1:1000) with human plasma lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu sérique humain (tissue serologique)
• Résultats positifs en IP: tissu plasmatique humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below

Informations sur le produit

21247-1-AP cible ITIH3 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ITIH3 Protéine recombinante Ag15750
• Nom complet: inter-alpha (globulin) inhibitor H3
• Masse moléculaire calculée: 890 aa, 100 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC107604
• Symbole du gène: ITIH3
• Identification du gène (NCBI): 3699
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ITIH3, or inter-alpha-trypsin inhibitor heavy chain 3, is a gene in humans that encodes the heavy chain subunit of the pre-alpha-trypsin inhibitor complex. This complex plays a role in stabilizing the extracellular matrix through its ability to bind hyaluronic acid. The gene is located on chromosome 3 and is part of the inter-alpha-trypsin inhibitor family gene cluster. ITIH3 has restricted expression primarily toward the liver, with an RPKM value of 228.8, indicating a relatively high level of expression in this organ.

Publications

Species	Application	Title
human	IHC	Br J Cancer; A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.; Authors - Xiaohui Liu
human	WB	J Pers Med; Comprehensive Analysis and Experimental Validation of a Novel Estrogen/Progesterone-Related Prognostic Signature for Endometrial Cancer.; Authors - Jing Yu
	WB	Sci Rep; Plasma Protein Comparison between Dairy Cows with Inactive Ovaries and Estrus.; Authors - Chang Zhao
human	IHC	Dis Markers; Plasma Inter-Alpha-Trypsin Inhibitor Heavy Chains H3 and H4 Serve as Novel Diagnostic Biomarkers in Human Colorectal Cancer.; Authors - Xiao Jiang

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