Anticorps Polyclonal de lapin anti-ITLN1/2

ITLN1/2 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(19)

N° de cat : 11770-1-AP

Synonymes

ITLN1, HL 1, hIntL, Galactofuranose-binding lectin, Galactofuranose binding lectin

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Galerie de données de validation

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  WB analysis of mouse colon using 11770-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11770-1-AP (ITLN1/2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse appendix using 11770-1-AP

  IP result of anti-ITLN1/2 (IP:11770-1-AP, 4ug; Detection:11770-1-AP 1:500) with mouse appendix tissue lysate 4000ug.

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  IHC staining of human stomach using 11770-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11770-1-AP (ITLN1/2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu de côlon de souris,
• Résultats positifs en IP: tissu d'appendice de souris
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 14 publications below
• IHC: See 5 publications below
• IF: See 8 publications below
• FC: See 1 publications below

Informations sur le produit

11770-1-AP cible ITLN1/2 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ITLN1/2 Protéine recombinante Ag2424
• Nom complet: intelectin 1 (galactofuranose binding)
• Masse moléculaire calculée: 313 aa, 35 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC020664
• Symbole du gène: ITLN1
• Identification du gène (NCBI): 55600
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ITLN1, also named as Intelectin 1, has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. ITLN1 is a glycoprotein made up of three 40 kDa subunits cross-linked by disulfide bonds making up a 120-kDa homo-trimer of 295 amino acids and N-linked oligosaccharides (PMID: 17621593). It may play a role in the defense system against microorganisms. Human intelectin-1 is expressed in intestinal goblet cells, and is present at about 200 ng/mL in serum. Intelectin-1 was also shown to be expressed in human endothelial cells and adipocytes of human abdominal adipose tissue. (PMID: 22768319,PMID: 28640441)

Publications

Species	Application	Title
	IF	Nat Commun; Tissue mechanics drives regeneration of a mucociliated epidermis on the surface of Xenopus embryonic aggregates.; Authors - Hye Young Kim
human	IHC, IF	J Exp Med; Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.; Authors - Yalong Wang
mouse	WB,IHC	Mucosal Immunol; Intelectin contributes to allergen-induced IL-25, IL-33, and TSLP expression and type 2 response in asthma and atopic dermatitis.; Authors - L Yi
mouse	WB,IF	Front Cell Dev Biol; Neuroprotective Effects of Omentin-1 Against Cerebral Hypoxia/Reoxygenation Injury via Activating GAS6/Axl Signaling Pathway in Neuroblastoma Cells.; Authors - Xiaochen Niu
human	WB	Fish Shellfish Immunol; Molecular properties and ligand specificity of zebrafish intelectin-2.; Authors - Nongnuch Singrang
human	WB	J Biol Chem; Structures of Xenopus embryonic epidermal lectin reveal a conserved mechanism of microbial glycan recognition.; Authors - Kittikhun Wangkanont

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