Anticorps Polyclonal de lapin anti-ITM2A

ITM2A Polyclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 18306-1-AP

Synonymes

BRICD2A, E25A, integral membrane protein 2A, ITM2A, Protein E25

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Galerie de données de validation

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  WB analysis using 18306-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18306-1-AP (ITM2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18306-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18306-1-AP (ITM2A antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  FC experiment of Jurkat using 18306-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human ITM2A (18306-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HepG2, cellules Jurkat
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• FC: See 1 publications below

Informations sur le produit

18306-1-AP cible ITM2A dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: integral membrane protein 2A
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC040437
• Symbole du gène: ITM2A
• Identification du gène (NCBI): 9452
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ITM2A, also named BRICD2A and E25A, is a member of type II integral membrane (ITM2) protein family. ITM2A expression is high in osteogenic and lymphoid tissues, while both ITM2B and ITM2C are expressed in brain. There are 5 articles specifically referring to this gene in PubMed. Proteins are expected to localize in various compartments (cytoplasm, integral to membrane). No phenotype has yet been reported to our knowledge: this gene's in vivo function is yet unknown.

Publications

Species	Application	Title
mouse	IHC	Bone Res; Single cell analysis reveals inhibition of angiogenesis attenuates the progression of heterotopic ossification in Mkx-/- mice.; Authors - Junxin Lin
human	FC	Blood; Identification of early gene expression changes during human Th17 cell differentiation.; Authors - Tuomela Soile S
human	WB,IHC,IF	Cell Commun Signal; Integral membrane protein 2A inhibits cell growth in human breast cancer via enhancing autophagy induction.; Authors - Cefan Zhou
human,mouse	IF	Fluids Barriers CNS; Exploring ITM2A as a new potential target for brain delivery.; Authors - Céline Cegarra
mouse	IF	iScience; The regenerating skeletal muscle niche drives satellite cell return to quiescence.; Authors - Alicia A Cutler
mouse	IHC	PLoS One; Itm2a expression in the developing mouse first lower molar, and the subcellular localization of itm2a in mouse dental epithelial cells.; Authors - Makiko Kihara

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C