Anticorps Polyclonal de lapin anti-ITPA

ITPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 16134-1-AP

Synonymes

Non-canonical purine NTP pyrophosphatase, My049, ITPase, HLC14 06 P, EC:3.6.1.9

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Galerie de données de validation

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  WB analysis of K-562 using 16134-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16134-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16134-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16134-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16134-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16134-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16134-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 16134-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16134-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16134-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16134-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16134-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16134-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16134-1-AP (ITPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 16134-1-AP

  IP result of anti-ITPA (IP:16134-1-AP, 4ug; Detection:16134-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human heart using 16134-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16134-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 16134-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16134-1-AP (ITPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16134-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 16134-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16134-1-AP (ITPA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 16134-1-AP

  Immunofluorescent analysis of MCF-7 cells, using ITPA antibody 11640-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa, cellules HT-1080, cellules NIH/3T3, tissu cardiaque de souris, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu cardiaque de souris, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

16134-1-AP cible ITPA dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ITPA Protéine recombinante Ag9128
• Nom complet: inosine triphosphatase (nucleoside triphosphate pyrophosphatase)
• Masse moléculaire calculée: 194 aa, 21 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC010138
• Symbole du gène: ITPA
• Identification du gène (NCBI): 3704
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ITPA(Inosine triphosphate pyrophosphatase) catalyzes the pyrophosphohydrolysis of inosine triphosphate (ITP) to inosine monophosphate (IMP).It expresses in the cytoplasm(PMID:11278832).It can be detected a band of 44 Kda as a homodimer in the western blot(PMID:19631656) though its predictedsize is 21kd.This protein has two isforms with the molecular weight of 20KDa and 21KDa.

Publications

Species	Application	Title
human	WB	Biochimie; Analysis of human ITPase nucleobase specificity by site-directed mutagenesis.; Authors - Hill Amanda A
human	IHC	Oncotarget; A four-gene signature predicts survival in clear-cell renal-cell carcinoma.; Authors - Jun Dai
human	WB	Biochim Biophys Acta; Quantitative in vitro and in vivo characterization of the human P32T mutant ITPase.; Authors - Herting Greg G

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