Anticorps Polyclonal de lapin anti-ITPR1-specific

ITPR1-specific Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

22

N° de cat : 19962-1-AP

Synonymes

INSP3R1, IP3 receptor isoform 1, IP3R, IP3R 1, IP3R1, ITPR1, SCA15, SCA16, Type 1 InsP3 receptor

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Galerie de données de validation

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  WB analysis of mouse brain using 19962-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19962-1-AP (ITPR1-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 19962-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19962-1-AP (ITPR1-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 19962-1-AP

  IP result of anti-ITPR1-specific (IP:19962-1-AP, 4ug; Detection:19962-1-AP 1:300) with mouse brain tissue lysate 3160 ug.

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  IHC staining of human brain using 19962-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 19962-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19962-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19962-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 19962-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 19962-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:100 (under 40x lens).

• 

  FC experiment of HepG2 using 19962-1-AP

  1X10^6 HepG2 cells were stained with 0.2 ug ITPR1-specific antibody (19962-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu hépatique de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral humain, tissu cérébral de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 19 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

19962-1-AP cible ITPR1-specific dans les applications de WB, IP, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, souris, duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: inositol 1,4,5-triphosphate receptor, type 1
• Masse moléculaire calculée: 314 kDa
• Poids moléculaire observé: 290-300 kDa
• Numéro d’acquisition GenBank: NM_001099952
• Symbole du gène: ITPR1
• Identification du gène (NCBI): 3708
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ITPR1, also named as INSP3R1 and IP3R, belongs to the InsP3 receptor family. It is a receptor for inositol 1,4,5-trisphosphate which is a second messenger that mediates the release of intracellular calcium. Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15). The antibody has no cross reaction with ITPR2 and ITPR3.

Publications

Species	Application	Title
human		Neuron; Nicotinamide Pathway-Dependent Sirt1 Activation Restores Calcium Homeostasis to Achieve Neuroprotection in Spinocerebellar Ataxia Type 7.; Authors - Colleen A Stoyas
mouse	WB	Mol Psychiatry; A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.; Authors - Yue Qin
mouse		Diabetes; PACS-2 Ameliorates Tubular Injury by Facilitating Endoplasmic Reticulum-Mitochondria Contact and Mitophagy in Diabetic Nephropathy.; Authors - Chenrui Li
human	WB	Cancer Lett; MORTALIN-Ca2+ axis drives innate rituximab resistance in diffuse large B-cell lymphoma.; Authors - Qi Sun
rat	WB	Mol Nutr Food Res; Sulforaphane Balances Ca2+ Homeostasis Injured by Excessive Fat via Mitochondria-Associated Membrane (MAM).; Authors - Sicong Tian
rat	WB	Front Immunol; Interleukin-6-Mediated-Ca2+ Handling Abnormalities Contributes to Atrial Fibrillation in Sterile Pericarditis Rats.; Authors - Jie Liao