Anticorps Polyclonal de lapin anti-Intersectin 1

Intersectin 1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 21862-1-AP

Synonymes

ITSN1, SH3 domain-containing protein 1A, ITSN 1, ITSN, Intersectin-1

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Galerie de données de validation

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  WB analysis of fetal human brain using 21862-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 21862-1-AP (Intersectin 1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 21862-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 21862-1-AP (Intersectin 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 21862-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 21862-1-AP (Intersectin 1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 21862-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 21862-1-AP (Intersectin 1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 21862-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 21862-1-AP (Intersectin 1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal
• Résultats positifs en IHC: tissu d'estomac humain, human intrahepatic cholangiocarcinoma tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

21862-1-AP cible Intersectin 1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Intersectin 1 Protéine recombinante Ag16517
• Nom complet: intersectin 1 (SH3 domain protein)
• Masse moléculaire calculée: 1721 aa, 195 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC116186
• Symbole du gène: Intersectin 1
• Identification du gène (NCBI): 6453
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Intersectin 1 (ITSN1) is a multidomain adaptor protein that plays a crucial role in various cellular processes, particularly in the nervous system. It is involved in clathrin-mediated endocytosis, signal transduction, and the regulation of the actin cytoskeleton. The protein is highly abundant in neurons and has been implicated in neurodevelopmental disorders, such as autism spectrum disorders, intellectual disability, and epilepsy. Recent studies have identified de novo variants in the ITSN1 gene in patients with these disorders, suggesting a link between ITSN1 and brain development.

Publications

Species	Application	Title
human	WB	Front Oncol; Liquid Biopsy of Extracellular Vesicle-Derived miR-193a-5p in Colorectal Cancer and Discovery of Its Tumor-Suppressor Functions.; Authors - Rui Wei

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