Anticorps Monoclonal anti-IVD

IVD Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2D12A5

N° de cat : 66032-1-Ig

Synonymes

Isovaleryl-CoA dehydrogenase, mitochondrial, EC:1.3.8.4, EC:1.3.8.1, Butyryl-CoA dehydrogenase, ACAD2

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Galerie de données de validation

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  WB analysis using 66032-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66032-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66032-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 66032-1-Ig

  human liver tissue were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66032-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66032-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66032-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66032-1-Ig(IVD antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 66032-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66032-1-Ig(IVD antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 66032-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66032-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 66032-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66032-1-Ig (IVD antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of human liver cancer using 66032-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66032-1-Ig (IVD antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of MCF-7 using 66032-1-Ig

  Immunofluorescent analysis of MCF-7 cells, using IVD antibody 66032-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat, cellules L02, cellules MCF-7, tissu hépatique humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du foie humain
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66032-1-Ig cible IVD dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IVD Protéine recombinante Ag18042
• Nom complet: isovaleryl Coenzyme A dehydrogenase
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 41-46 kDa
• Numéro d’acquisition GenBank: BC017202
• Symbole du gène: IVD
• Identification du gène (NCBI): 3712
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IVD(Isovaleryl CoA dehydrogenase, mitochondrial) is a member of the acylCoA dehydrogenase family and is involved in the catabolism of leucine. In size, IVD precursor and mature proteins produced by class I mutants are indistinguishable from their normal counterparts. Class II, III, and IV mutants make IVD precursor proteins 42 kD in size rather than the normal 45 kD(PMID:2063866). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
rat	WB	Arch Toxicol; Activation of peroxisome proliferator-activated receptor α ameliorates perfluorododecanoic acid-induced production of reactive oxygen species in rat liver.; Authors - Hui Liu

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