Anticorps Polyclonal de lapin anti-IVNS1ABP

IVNS1ABP Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 14741-1-AP

Synonymes

Kelch-like protein 39, Influenza virus NS1A-binding protein, HSPC068, FLARA3, Aryl hydrocarbon receptor-associated protein 3

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Galerie de données de validation

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  WB analysis of mouse heart using 14741-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 14741-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14741-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14741-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Apoptosised HeLa using 14741-1-AP

  Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 14741-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14741-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14741-1-AP (IVNS1ABP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 14741-1-AP

  IP result of anti-IVNS1ABP (IP:14741-1-AP, 4ug; Detection:14741-1-AP 1:800) with mouse heart tissue lysate 4000ug.

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  IHC staining of human kidney using 14741-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14741-1-AP (IVNS1ABP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14741-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14741-1-AP (IVNS1ABP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 14741-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14741-1-AP (IVNS1ABP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14741-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14741-1-AP (IVNS1ABP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse kidney using 14741-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14741-1-AP (IVNS1ABP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse kidney using 14741-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14741-1-AP (IVNS1ABP Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HEK-293, cellules HeLa, cellules HeLa apoptosées, cellules K-562, tissu ovarien de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

14741-1-AP cible IVNS1ABP dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IVNS1ABP Protéine recombinante Ag6412
• Nom complet: influenza virus NS1A binding protein
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC067739
• Symbole du gène: IVNS1ABP
• Identification du gène (NCBI): 10625
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IVNS1ABP is involved in many cell functions, including pre-mRNA splicing, the aryl hydrocarbon receptor (AHR) pathway, F-actin organization and protein ubiquitination. It may play a role in mRNA nuclear export.

Publications

Species	Application	Title
human	WB	Nature; Global landscape of HIV-human protein complexes.; Authors - Stefanie Jäger
human,mouse	WB	Inflammation; Nonstructural Protein 1 Mediates HMGB1 Release by Targeting Histone H1.0 After Respiratory Syncytial Virus Infection In Vivo and In Vitro; Authors - Na Zhou

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