Anticorps Polyclonal de lapin anti-IWS1

IWS1 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 16943-1-AP

Synonymes

IWS1, IWS1 homolog (S. cerevisiae), IWS1 like protein, IWS1L, Protein IWS1 homolog

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Galerie de données de validation

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  WB analysis using 16943-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16943-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16943-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 16943-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16943-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16943-1-AP

  IP Result of anti-IWS1 (IP:16943-1-AP, 4ug; Detection:16943-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• ChIP: See 1 publications below

Informations sur le produit

16943-1-AP cible IWS1 dans les applications de WB, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IWS1 Protéine recombinante Ag10554
• Nom complet: IWS1 homolog (S. cerevisiae)
• Masse moléculaire calculée: 819 aa, 92 kDa
• Poids moléculaire observé: 116-120 kDa
• Numéro d’acquisition GenBank: BC017012
• Symbole du gène: IWS1
• Identification du gène (NCBI): 55677
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
	ChIP	Cell Host Microbe; The Integrase Cofactor LEDGF/p75 Associates with Iws1 and Spt6 for Postintegration Silencing of HIV-1 Gene Expression in Latently Infected Cells.; Authors - Annabelle Gérard

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sarah (Verified Customer) (12-31-2020)

Works perfectly in WB and immunoprecipitation experiements (HeLa and HEL293T cells).

• Applications: Western Blot, Immunoprecipitation, ChIP
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa and HEK293T