Anticorps Polyclonal de lapin anti-Icam-1

• Phare
• Validé par KD/KO

Icam-1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris et plus (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

71

Avis (4)

N° de cat : 10020-1-AP

Synonymes

CD54, ICAM 1, Icam1, Icam-1, Ly 47, MALA 2

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Galerie de données de validation

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  WB analysis of mouse brain using 10020-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10020-1-AP (Icam-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10020-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10020-1-AP (Icam-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10020-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10020-1-AP (Icam-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 10020-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10020-1-AP (Icam-1 antibody) at dilution of 1:6400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10020-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10020-1-AP (Icam-1 antibody) at dilution of 1:6400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 10020-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10020-1-AP (Icam-1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse liver using 10020-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10020-1-AP (Icam-1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse liver using 10020-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Icam-1 antibody (10020-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse liver using 10020-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Icam-1 antibody (10020-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu hépatique de souris, tissu splénique de souris
• Résultats positifs en IHC: tissu pulmonaire de souris, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu hépatique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:3200-1:12800
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 47 publications below
• IHC: See 18 publications below
• IF: See 18 publications below
• FC: See 2 publications below

Informations sur le produit

10020-1-AP cible Icam-1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat, bovin, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: intercellular adhesion molecule 1
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 95-100 kDa
• Numéro d’acquisition GenBank: NM_010493
• Symbole du gène: Icam1
• Identification du gène (NCBI): 15894
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Where is ICAM-1 expressed?

Intercellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54) is a transmembrane glycoprotein constitutively expressed at low levels in endothelial cells, pericytes and on some lymphocytes and monocytes¹. It is located at the cytoplasmic membrane, with a large extracellular region of mainly hydrophobic amino acids joined to a small transmembrane region and a cytoplasmic tail. It has a molecular weight of 75 to 115 kDa depending on the level of glycosylation.

 What is the function of ICAM-1?

ICAM-1 is important in both innate and adaptive immune responses as an adhesion molecule. Although it is constitutively expressed, in the presence of pro-inflammatory cytokines such as TNFα the endothelial cells are activated and upregulate expression of ICAM-1². In blood vessels lined with endothelial cells, leukocytes that are rolling over the surface are able to bind to ICAM-1 and transmigrate through the endothelial barrier and into the tissue. The initial binding of the leukocytes to ICAM-1 causes a Ca2+ release that initiates endothelial cell contraction and weakening of the intercellular tight junctions^{3, 4}. This protein can be used as an indicator of endothelial activation and of vascular inflammation.

 What is the role of ICAM-1 in disease?

Beyond the role in the immune response, ICAM-1 has also been identified as the target of attachment for the human rhinovirus, the cause of the common cold. Binding of the virus to ICAM-1 causes the viral capsid to uncoat and leads to release of the genetic material⁵.

1.  Hubbard, A. K. & Rothlein, R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radic. Biol. Med. 28, 1379-86 (2000).

2. Long, E. O. ICAM-1: getting a grip on leukocyte adhesion. J. Immunol. 186, 5021-3 (2011).

3. Lawson, C. & Wolf, S. ICAM-1 signaling in endothelial cells. (2009).

4. Lyck, R. & Enzmann, G. The physiological roles of ICAM-1 and ICAM-2 in neutrophil migration into tissues. Curr. Opin. Hematol. 22, 53-59 (2015).

5. Xing, L., Casasnovas, J. M. & Cheng, R. H. Structural analysis of human rhinovirus complexed with ICAM-1 reveals the dynamics of receptor-mediated virus uncoating. J. Virol. 77, 6101-7 (2003).

Publications

Species	Application	Title
mouse	WB	Immunity; Toll-like receptor 4 signaling by follicular dendritic cells is pivotal for germinal center onset and affinity maturation.; Authors - Alexandre Garin
mouse	IF	Nature; Mechanosensing by β1 integrin induces angiocrine signals for liver growth and survival.; Authors - Linda Lorenz
mouse	IF	Gastroenterology; ECM1 Prevents Activation of Transforming Growth Factor β, Hepatic Stellate Cells, and Fibrogenesis in Mice.; Authors - Weiguo Fan
		Hepatology; Angiocrine Wnt signaling controls liver growth and metabolic maturation in mice.; Authors - Thomas Leibing
mouse	WB	Nat Commun; Reducing Akt2 in retinal pigment epithelial cells causes a compensatory increase in Akt1 and attenuates diabetic retinopathy; Authors - Haitao Liu
	IF	EBioMedicine; Patient-derived melanoma organoid models facilitate the assessment of immunotherapies; Authors - Lingling Ou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iram (Verified Customer) (06-13-2022)

Very good antibody for immunoblotting

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: colon cancer cells

FH

Balawant (Verified Customer) (05-08-2022)

This antibody I have used from the total cell lysate of pancreatic tissue from the mice tissue . this antibody is working good.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: pancreatic tissue of mice

FH

Susmita (Verified Customer) (03-11-2022)

This antibody wonderfully works for my WB.

• Applications: Western Blot
• Primary Antibody Dilution: WB: 1: 2000
• Cell Tissue Type: CRC cell line

FH

Alice (Verified Customer) (08-21-2020)

We use this primer antibody to do IHC staining (Post mouse lung transfer surgery) .

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: lung from Mouse