Anticorps Polyclonal de lapin anti-Rabbit IgG control
Rabbit IgG control Polyclonal Antibody for FC, IP, WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Applications
WB, RIP, IP, IHC, FC, CoIP, ChIP, Cell treatment, ELISA
Conjugaison
Non conjugué
241
N° de cat : 30000-0-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | Protéine, Contrôle |
| Résultats positifs en IP | Contrôle |
| Résultats positifs en cytométrie | cellules Jurkat, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Informations sur le produit
30000-0-AP cible Rabbit IgG control dans les applications de WB, RIP, IP, IHC, FC, CoIP, ChIP, Cell treatment, ELISA et montre une réactivité avec des échantillons
| Réactivité | |
| Réactivité citée | rat, bovin, Humain, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | s/o |
| Nom complet | Rabbit IgG control |
| Symbole du gène | |
| Identification du gène (NCBI) | |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Protein A affinity purification |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Publications
| Species | Application | Title |
|---|---|---|
Drug Resist Updat CHD4 promotes acquired chemoresistance and tumor progression by activating the MEK/ERK axis | ||
Cell Res Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity | ||
ACS Nano Augmenting Intracellular Cargo Delivery of Extracellular Vesicles in Hypoxic Tissues through Inhibiting Hypoxia-Induced Endocytic Recycling | ||
Mol Cell CPT1A induction following epigenetic perturbation promotes MAVS palmitoylation and activation to potentiate antitumor immunity | ||
Mol Cell Interaction of tau with HNRNPA2B1 and N6-methyladenosine RNA mediates the progression of tauopathy. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Siddharth (Verified Customer) (08-02-2023) | Very efficient secondary antibody. Can be used effectively at 1:10,000 dilution.
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FH Macarena (Verified Customer) (10-07-2022) | very concentrated. Excellent results. Deffinetly use less than you would normally use.
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FH Guo-rong (Verified Customer) (08-30-2022) | It works well as an antibody control in neutralization experiments
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FH Anita (Verified Customer) (01-04-2022) | Works well as a control for IP
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FH Kenneth (Verified Customer) (02-14-2019) | Great company with Great products. Highly recommended.
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FH Priyanka (Verified Customer) (12-18-2018) |
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FH Seongjin (Verified Customer) (12-14-2018) |
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