Anticorps Polyclonal de lapin anti-IL-1 beta

IL-1 beta Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(211)

Avis (1)

N° de cat : 26048-1-AP

Synonymes

IL 1beta, Il1b, IL 1 beta, Il 1b, IL-1 beta,IL 1 beta,IL1beta

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Galerie de données de validation

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  WB analysis using 26048-1-AP

  LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 26048-1-AP (IL-1 beta antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26048-1-AP

  LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26048-1-AP (IL-1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26048-1-AP

  Untreated, LPS and Brefeldin A treated NR8383 cells were subjected to SDS PAGE followed by western blot with 26048-1-AP (IL-1 beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, LPS et à la bréfeldine A

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 162 publications below
• IHC: See 38 publications below
• IF: See 22 publications below
• ELISA: See 1 publications below

Informations sur le produit

26048-1-AP cible IL-1 beta dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-1 beta Protéine recombinante Ag23461
• Nom complet: interleukin 1 beta
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: NM_008361
• Symbole du gène: IL-1 beta
• Identification du gène (NCBI): 16176
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets.

Publications

Species	Application	Title
human	WB	Nature; Recognition and maturation of IL-18 by caspase-4 noncanonical inflammasome; Authors - Xuyan Shi
rat	IHC	Signal Transduct Target Ther; Metabolic reprogramming of proinflammatory macrophages by target delivered roburic acid effectively ameliorates rheumatoid arthritis symptoms; Authors - Na Jia
mouse	IHC	Bioact Mater; A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds; Authors - Shunlai Shang
human,rat	WB	ACS Nano; Hepatic Stellate Cell- and Liver Microbiome-Specific Delivery System for Dihydrotanshinone I to Ameliorate Liver Fibrosis; Authors - Xia Niu
mouse	IHC	Adv Sci (Weinh); Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis; Authors - Bin Liu
mouse	WB	Acta Pharm Sin B; Ligand-based substituent-anchoring design of selective receptor-interacting protein kinase 1 necroptosis inhibitors for ulcerative colitis therapy; Authors - Jing Zhu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marina (Verified Customer) (06-29-2021)

I detected pro-il1b from lysate of macrophages using a dilution of 1:1000, and exposing the membrane for 30sec. Really good and specific antibody. I strongly recommend

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Macrophages

IL-1 beta Antibody Western Blot validation (1:1000 dilution) in Macrophages (Cat no:26048-1-AP)

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