Anticorps Polyclonal de lapin anti-IL-1 beta

IL-1 beta Polyclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris et plus (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

76

Avis (1)

N° de cat : 26048-1-AP

Synonymes

Il 1b, IL 1beta, IL-1 beta, Il1b, IL-1B, interleukin 1 beta

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Galerie de données de validation

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  WB analysis using 26048-1-AP

  LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26048-1-AP (IL-1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26048-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26048-1-AP (IL-1 beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  FC experiment of RAW 264.7 using 26048-1-AP

  1X10^6 RAW 264.7 cells untreated (dashed lines) and LPS and Brefeldin A treated (red) were intracellularly stained with 0.4 ug Anti-Mouse IL-1 beta (26048-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.4 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of RAW 264.7 using 26048-1-AP

  1X10^6 RAW 264.7 cells untreated and LPS and Brefeldin A treated were intracellularly stained with 0.4 ug Anti-Mouse IL-1 beta (26048-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7,
• Résultats positifs en cytométrie: cellules RAW 264.7 traitées au LPS et à la bréfeldine A,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 60 publications below
• IHC: See 11 publications below
• IF: See 6 publications below
• ELISA: See 1 publications below

Informations sur le produit

26048-1-AP cible IL-1 beta dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-1 beta Protéine recombinante Ag23461
• Nom complet: interleukin 1 beta
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: NM_008361
• Symbole du gène: Il1b
• Identification du gène (NCBI): 16176
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets.

Publications

Species	Application	Title
human	WB	Nature; Recognition and maturation of IL-18 by caspase-4 noncanonical inflammasome; Authors - Xuyan Shi
rat	IHC	Signal Transduct Target Ther; Metabolic reprogramming of proinflammatory macrophages by target delivered roburic acid effectively ameliorates rheumatoid arthritis symptoms; Authors - Na Jia
human,rat	WB	ACS Nano; Hepatic Stellate Cell- and Liver Microbiome-Specific Delivery System for Dihydrotanshinone I to Ameliorate Liver Fibrosis; Authors - Xia Niu
mouse	WB	Acta Pharm Sin B; Ligand-based substituent-anchoring design of selective receptor-interacting protein kinase 1 necroptosis inhibitors for ulcerative colitis therapy; Authors - Jing Zhu
rat	WB,IF	J Control Release; Controlled extracellular vesicles release from aminoguanidine nanoparticle-loaded polylysine hydrogel for synergistic treatment of spinal cord injury; Authors - Shaoke Wang
mouse	IF	Acta Biomater; ROS-responsive magnesium-containing microspheres for antioxidative treatment of intervertebral disc degeneration; Authors - Tianhui Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marina (Verified Customer) (06-29-2021)

I detected pro-il1b from lysate of macrophages using a dilution of 1:1000, and exposing the membrane for 30sec. Really good and specific antibody. I strongly recommend

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Macrophages

IL-1 beta Antibody Western Blot validation (1:1000 dilution) in Macrophages (Cat no:26048-1-AP)