Anticorps Monoclonal anti-Integrin alpha 1

Integrin alpha 1 Monoclonal Antibody for WB, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

1C4F2

N° de cat : 68211-1-Ig

Synonymes

ITGA1, Laminin and collagen receptor, integrin, alpha 1, CD49a, CD49 antigen-like family member A

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Galerie de données de validation

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  WB analysis of HepG2 using 68211-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68211-1-Ig (Integrin alpha-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 68211-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68211-1-Ig (Integrin alpha-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of human placenta using 68211-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Integrin alpha 1 antibody (68211-1-Ig, Clone: 1C4F2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 68211-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Integrin alpha 1 antibody (68211-1-Ig, Clone: 1C4F2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu placentaire humain
• Résultats positifs en IF-P: tissu placentaire humain,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68211-1-Ig cible Integrin alpha 1 dans les applications de WB, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Integrin alpha 1 Protéine recombinante Ag30631
• Nom complet: integrin, alpha 1
• Masse moléculaire calculée: 1179 aa, 131 kDa
• Poids moléculaire observé: 180 kDa
• Numéro d’acquisition GenBank: BC137121
• Symbole du gène: Integrin alpha 1
• Identification du gène (NCBI): 3672
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).

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