Anticorps Polyclonal de lapin anti-Integrin alpha 6

• Phare
• Validé par KD/KO

Integrin alpha 6 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(27)

Avis (1)

N° de cat : 27189-1-AP

Synonymes

ITGA6, CD49 antigen-like family member F, CD49f, Integrin alpha-6, Integrin alpha-6 heavy chain

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Galerie de données de validation

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  WB analysis of A549 using 27189-1-AP

  WB result of Integrin Alpha 6 antibody (27189-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 6 transfected A549 cells.

• 

  WB analysis using 27189-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27189-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27189-1-AP

  WB result of Integrin Alpha 6 antibody (27189-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 6 transfected HepG2 cells.

• 

  WB analysis of A431 using 27189-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skin using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27189-1-AP (Integrin alpha 6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A431, cellules HepG2, cellules SW480, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu cutané de souris, tissu de côlon de souris, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 17 publications below
• IHC: See 6 publications below
• IF: See 10 publications below

Informations sur le produit

27189-1-AP cible Integrin alpha 6 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, singe, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Integrin alpha 6 Protéine recombinante Ag25835
• Nom complet: integrin, alpha 6
• Masse moléculaire calculée: 1073 aa, 119 kDa
• Poids moléculaire observé: 110-130 kDa
• Numéro d’acquisition GenBank: BC136456
• Symbole du gène: Integrin alpha 6
• Identification du gène (NCBI): 3655
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha 6 (ITGA6, also known as CD49f or VLA-6) is a 120-kDa single-pass type 1 transmembrane glycoprotein, which combines with the beta 1 subunit (ITGB1, CD29) or beta 4 subunit (ITGB4, CD104) to form heterodimeric laminin receptors (PMID: 1976638; 2351695). Integrin alpha 6 mediates cell-to-cell and cell-to-stroma adhesion. It has also been found in many different stem cell populations and is involved in homing and connecting stem cells to their niches and regulating self-renewal mechanisms in stem cells (PMID: 28494695).

Publications

Species	Application	Title
human	WB	ACS Appl Mater Interfaces; A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells; Authors - Xinyang Zhao
bovine	IF	Cells; Comparative Analysis of mRNA and miRNA Expression between Dermal Papilla Cells and Hair Matrix Cells of Hair Follicles in Yak; Authors - Xiaolan Zhang
monkey	WB	Virulence; Role of intestinal extracellular matrix-related signaling in porcine epidemic diarrhea virus infection.; Authors - Yuchen Li
human	WB	Stem Cell Res Ther; Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.; Authors - Ling Leng
Hamster	IF	J Virol; Single-cell analysis of the impact of host-cell heterogeneity on infection with foot and mouth disease virus.; Authors - Xiu Xin
rat	IF	Vascul Pharmacol; Midkine mediates dysfunction of liver sinusoidal endothelial cells through integrin α4 and α6; Authors - Li Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Insa (Verified Customer) (03-22-2023)

Did work very well in ICC

• Applications: Other
• Primary Antibody Dilution: 1:100-1:400
• Cell Tissue Type: iPSC derived Astrocytes

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