Anticorps Polyclonal de lapin anti-Integrin Alpha 6

• Phare
• Validé par KD/KO

Integrin Alpha 6 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

19

Avis (1)

N° de cat : 27189-1-AP

Synonymes

CD49f, Integrin alpha 6, Integrin alpha-6, integrin, alpha 6, ITGA6, ITGA6B, VLA 6

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Galerie de données de validation

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  WB analysis of A549 using 27189-1-AP

  WB result of Integrin Alpha 6 antibody (27189-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 6 transfected A549 cells.

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  WB analysis using 27189-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27189-1-AP

  WB result of Integrin Alpha 6 antibody (27189-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 6 transfected HepG2 cells.

• 

  WB analysis of A431 using 27189-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 27189-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin Alpha 6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 27189-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 27189-1-AP (Integrin Alpha 6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skin using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27189-1-AP (Integrin Alpha 6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27189-1-AP (Integrin Alpha 6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 27189-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27189-1-AP (Integrin alpha-6 Antibody) at dilution of 1:200 (under 40x lens).

• 

  FC experiment of A431 using 27189-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Integrin Alpha 6 (27189-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A431, cellules HepG2, tissu cutané de souris, tissu pulmonaire de rat
• Résultats positifs en IHC: tissu cutané de souris, tissu de côlon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 6 publications below
• IF: See 6 publications below

Informations sur le produit

27189-1-AP cible Integrin Alpha 6 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, singe, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Integrin Alpha 6 Protéine recombinante Ag25835
• Nom complet: integrin, alpha 6
• Masse moléculaire calculée: 1073 aa, 119 kDa
• Poids moléculaire observé: 110-130 kDa
• Numéro d’acquisition GenBank: BC136456
• Symbole du gène: ITGA6
• Identification du gène (NCBI): 3655
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	ACS Appl Mater Interfaces; A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells; Authors - Xinyang Zhao
bovine	IF	Cells; Comparative Analysis of mRNA and miRNA Expression between Dermal Papilla Cells and Hair Matrix Cells of Hair Follicles in Yak; Authors - Xiaolan Zhang
monkey	WB	Virulence; Role of intestinal extracellular matrix-related signaling in porcine epidemic diarrhea virus infection.; Authors - Yuchen Li
human	WB	Stem Cell Res Ther; Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.; Authors - Ling Leng
Hamster	IF	J Virol; Single-cell analysis of the impact of host-cell heterogeneity on infection with foot and mouth disease virus.; Authors - Xiu Xin
rat	IF	Vascul Pharmacol; Midkine mediates dysfunction of liver sinusoidal endothelial cells through integrin α4 and α6; Authors - Li Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Insa (Verified Customer) (03-22-2023)

Did work very well in ICC

• Applications: Other
• Primary Antibody Dilution: 1:100-1:400
• Cell Tissue Type: iPSC derived Astrocytes

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C