Anticorps Monoclonal anti-Integrin Beta 1/CD29

Integrin Beta 1/CD29 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1A7C9

Avis (1)

N° de cat : 66315-1-Ig

Synonymes

ITGB1, 1A7C9, CD29, FNRB, Glycoprotein IIa

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Galerie de données de validation

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  WB analysis of HT-1080 using 66315-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 66315-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 66315-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules HUVEC

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

66315-1-Ig cible Integrin Beta 1/CD29 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Integrin Beta 1/CD29 Protéine recombinante Ag24476
• Nom complet: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC020057
• Symbole du gène: Integrin beta 1
• Identification du gène (NCBI): 3688
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

Publications

Species	Application	Title
human	WB	Structure; Phosphorylation of RIAM by Src Promotes Integrin Activation by Unmasking the PH Domain of RIAM.; Authors - Eun-Ah Cho
rat	WB	Front Med (Lausanne); Low hydrostatic pressure promotes functional homeostasis of nucleus pulposus cells through the TRPV4/CRT/FA complex axis; Authors - Junxian Hu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ryan (Verified Customer) (01-24-2018)

Antigen retrieval - Tris HCl 0.1M ph 9

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish Retina/Brain

Integrin Beta 1/CD29 Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish Retina/Brain (Cat no:66315-1-Ig)

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