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Anticorps Monoclonal anti-Integrin Beta 1

Integrin Beta 1 Monoclonal Antibody for FC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

1A7C9

N° de cat : 66315-1-Ig

Synonymes

CD29, FNRB, GPIIA, Integrin beta 1, Integrin beta-1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB

Galerie de données de validation

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  • Western Blot (WB) analysis of HT-1080 cells using Integrin Beta 1 Monoclonal antibody (66315-1-Ig)

    WB analysis of HT-1080 using 66315-1-Ig

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HUVEC cells using Integrin Beta 1 Monoclonal antibody (66315-1-Ig)

    WB analysis of HUVEC using 66315-1-Ig

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using Integrin Beta 1 Monoclonal antibody (66315-1-Ig)

    WB analysis of HT-1080 using 66315-1-Ig

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HUVEC cells using Integrin Beta 1 Monoclonal antibody (66315-1-Ig)

    FC experiment of HUVEC using 66315-1-Ig

    1X10^6 HUVEC cells were stained with 0.20 ug Anti-Human Integrin beta-1 (66315-1-Ig, Clone:1A7C9) (red) or 0.20 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone:T1F8D3F10) (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Cells were fixed with 90% MeOH.

Applications testées

Résultats positifs en WBcellules HT-1080, cellules HUVEC
Résultats positifs en cytométriecellules HUVEC

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

WBSee 1 publications below

Informations sur le produit

66315-1-Ig cible Integrin Beta 1 dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Integrin Beta 1 Protéine recombinante Ag24476
Nom complet integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Masse moléculaire calculée 88 kDa
Poids moléculaire observé 130 kDa
Numéro d’acquisition GenBankBC020057
Symbole du gène ITGB1
Identification du gène (NCBI) 3688
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

Protocole

Product Specific Protocols
WB protocol for Integrin Beta 1 antibody 66315-1-IgDownload protocol
FC protocol for Integrin Beta 1 antibody 66315-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Structure

Phosphorylation of RIAM by Src Promotes Integrin Activation by Unmasking the PH Domain of RIAM.

Authors - Eun-Ah Cho
View Article

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Ryan (Verified Customer) (01-24-2018)

Antigen retrieval - Tris HCl 0.1M ph 9

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish Retina/Brain
Integrin Beta 1 Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish Retina/Brain (Cat no:66315-1-Ig)