Anticorps Polyclonal de lapin anti-IFN-beta

IFN-beta Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(20)

N° de cat : 27506-1-AP

Synonymes

IFN beta, IFNB1, IFN β1, IFNB, IFN-β1

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Galerie de données de validation

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  WB analysis of Recombinant protein using 27506-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 27506-1-AP (IFN Beta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 27506-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IFN-beta antibody (27506-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  IF Staining of L02 using 27506-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using IFN-beta antibody (27506-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 27506-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 27506-1-AP (IFN-beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: Protéine recombinante,
• Résultats positifs en IF/ICC: cellules HepG2, cellules L02

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 7 publications below
• IF: See 3 publications below

Informations sur le produit

27506-1-AP cible IFN-beta dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFN-beta Protéine recombinante Ag26326
• Nom complet: IFN beta
• Masse moléculaire calculée: 187 aa, 22 kDa
• Numéro d’acquisition GenBank: BC069314
• Symbole du gène: IFN beta1
• Identification du gène (NCBI): 3456
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interferon-beta (IFN-β) is a type I interferon, which is a group of signaling proteins made and released by host cells in response to the presence of several viruses, including double-stranded RNA produced by many viruses. IFN-β is a single protein species and is molecularly related to IFN-α subtypes, although it is antigenically distinct from them. It is mainly produced by fibroblasts and plays a crucial role in the innate immune response against viruses. IFN-β can inhibit viral replication by interfering with the virus's RNA or DNA, thus suppressing viral growth. It also enhances the cytotoxic activity of natural killer (NK) cells and promotes the expression of major histocompatibility complex (MHC) class I molecules.

Publications

Species	Application	Title
human	IHC	J Mater Chem B; Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating; Authors - Bin Yan
human	WB	Int Immunopharmacol; Polysaccharides from Platycodonis Radix ameliorated respiratory syncytial virus-induced epithelial cell apoptosis and inflammation through activation of miR-181a-mediated Hippo and SIRT1 pathways.; Authors - Juan-Juan Li
human	WB	Amino Acids; Escherichia coli infection activates the production of IFN-α and IFN-β via the JAK1/STAT1/2 signaling pathway in lung cells.; Authors - Yan Jin
human	WB	BMC Vet Res; The role of IBV PL1pro in virus replication and suppression of host innate immune responses; Authors - Weirong Liu
human	IF	Cell Death Dis; Intracellular virus sensor MDA5 exacerbates vitiligo by inducing the secretion of chemokines in keratinocytes under virus invasion.; Authors - Tongtian Zhuang
		Virology; Role of heparan sulfate in the Zika virus entry, replication, and cell death.; Authors - Huixin Gao

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