Anticorps Polyclonal de lapin anti-JIP1/IB-1

JIP1/IB-1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 14568-1-AP

Synonymes

MAPK8IP1, Islet-brain 1, Islet brain 1, IB-1, IB1

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Galerie de données de validation

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  WB analysis of fetal human brain using 14568-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 14568-1-AP (JIP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14568-1-AP

  IP result of anti-JIP1/IB-1 (IP:14568-1-AP, 4ug; Detection:14568-1-AP 1:500) with mouse brain tissue lysate 1840 ug.

• 

  IP experiment of rat brain using 14568-1-AP

  IP result of anti-JIP1/IB-1 (IP:14568-1-AP, 4ug; Detection:14568-1-AP 1:500) with rat brain tissue lysate 1120 ug.

• 

  IHC staining of human oesophagus cancer using 14568-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 14568-1-AP (JIP1/IB-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 14568-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 14568-1-AP (JIP1/IB-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14568-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14568-1-AP (JIP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 14568-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14568-1-AP (JIP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 14568-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14568-1-AP (JIP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 14568-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14568-1-AP (JIP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human oesophagus cancer using 14568-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 14568-1-AP (JIP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus cancer using 14568-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 14568-1-AP (JIP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of PC-12 using 14568-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using JIP1/IB-1 antibody (14568-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal
• Résultats positifs en IP: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu de cancer de l'œsophage humain, tissu cérébral de souris, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-12,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

14568-1-AP cible JIP1/IB-1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: JIP1/IB-1 Protéine recombinante Ag6150
• Nom complet: mitogen-activated protein kinase 8 interacting protein 1
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 97-116 kDa
• Numéro d’acquisition GenBank: BC068470
• Symbole du gène: MAPK8IP1/IB-1
• Identification du gène (NCBI): 9479
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Curr Biol; Stretch triggers microtubule stabilization and MARCKS-dependent membrane incorporation in the shaft of embryonic axons; Authors - Sara C Sousa
mouse	IF	ACS Chem Neurosci; Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.; Authors - Lei Wu
human	IP, WB	Front Physiol; Ras-Related C3 Botulinum Toxin Substrate 1 Combining With the Mixed Lineage Kinase 3- Mitogen-Activated Protein Kinase 7- c-Jun N-Terminal Kinase Signaling Module Accelerates Diabetic Nephropathy.; Authors - Changjiang Ying
mouse	WB	Saudi Pharm J; iTRAQ-derived quantitative proteomics uncovers the neuroprotective property of bexarotene in a mice model of cerebral ischemia-reperfusion injury.; Authors - Hailin Liu
mouse	WB	Cell Rep; SFRS11 Loss Leads to Aging-Associated Cognitive Decline by Modulating LRP8 and ApoE.; Authors - Obayed Raihan
human	WB	Oncotarget; Autophagy-related gene expression is an independent prognostic indicator of glioma.; Authors - Huixue Zhang

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