Anticorps Polyclonal de lapin anti-KANK2

KANK2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 21733-1-AP

Synonymes

ANKRD25, KANK2, KIAA1518, MXRA3, SIP, SRC 1 interacting protein, SRC1 interacting protein

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Galerie de données de validation

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  WB analysis using 21733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21733-1-AP (KANK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 21733-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 21733-1-AP (KANK2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 21733-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21733-1-AP (KANK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 21733-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21733-1-AP (KANK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 21733-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21733-1-AP (KANK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21733-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using KANK2 antibody (21733-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 21733-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using KANK2 antibody (21733-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 B23/NPM1 antibody (CL594-60096, Clone: 4F12A3, red).

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  IF Staining of A549 using 21733-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using KANK2 antibody (21733-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HEK-293 transfectées, tissu pulmonaire de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

21733-1-AP cible KANK2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KANK2 Protéine recombinante Ag16416
• Nom complet: KN motif and ankyrin repeat domains 2
• Masse moléculaire calculée: 851 aa, 91 kDa
• Poids moléculaire observé: 95-100 kDa
• Numéro d’acquisition GenBank: BC098105
• Symbole du gène: KANK2
• Identification du gène (NCBI): 25959
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Involved in transcription regulation by sequestering nuclear receptor coactivators, such as NCOA1, NCOA2 and NCOA3, in the cytoplasm; the function is deregulated by phosphorylation. May be involved in the control of cytoskeleton formation by regulating actin polymerization. Involved in regulation of caspase-independent apoptosis; proposed to sequester AIFM1 in mitochondria and apoptotic stimuli lead to its proteasomal degradation allowing the release of AIFM1 to the nucleus. May be involved in promotion of cell proliferation.

Publications

Species	Application	Title
mouse	WB,IF	J Cell Biol; The RNA-binding protein Fus directs translation of localized mRNAs in APC-RNP granules.; Authors - Yasuda Kyota K
human	WB	J Biol Chem; Nephrotic syndrome-associated mutation of KANK2 induces pathologic binding competition with physiological interactor KIF21A.; Authors - Yuqun Xu
mouse	WB	Am J Physiol Cell Physiol; Knockout of the neonatal Fc Receptor (FcRn) in cultured podocytes alters IL-6 signaling and the actin cytoskeleton.; Authors - Pantipa Tonsawan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Bryce (Verified Customer) (09-12-2022)

Strong band at expected molecular weight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000