Anticorps Polyclonal de lapin anti-KANSL3

KANSL3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 25533-1-AP

Synonymes

KIAA1310, KAT8 regulatory NSL complex subunit 3, PRTD, NSL3, NSL complex protein NSL3

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Galerie de données de validation

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  WB analysis using 25533-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25533-1-AP (KANSL3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 25533-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25533-1-AP (KANSL3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 25533-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25533-1-AP (KANSL3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat, cellules U2OS
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

25533-1-AP cible KANSL3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KANSL3 Protéine recombinante Ag22117
• Nom complet: KIAA1310
• Masse moléculaire calculée: 878 aa, 93 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC063792
• Symbole du gène: KANSL3
• Identification du gène (NCBI): 55683
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KANSL3, or KAT8-associated nonspecific lethal complex subunit 3, is a component of the KANSL complex, which is an important chromatin modifier in higher animals. This complex plays a crucial role in regulating housekeeping genes and is highly conserved across species. KANSL3, along with other KANSL complex members, is essential for cell division and has been shown to interact robustly both in vitro and in vivo. KANSL3 has been found to have a dual role in cellular function. In interphase, KANSL3 is chromatin-bound and regulates the expression of housekeeping genes. During mitosis, KANSL3 relocates to the mitotic spindle and is important for cell division. It has been identified as a microtubule minus-end-binding protein, which is a novel function for a chromatin-binding complex. KANSL3's ability to bind directly to microtubule minus ends is significant, as very few proteins are known to perform this function.

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