Anticorps Polyclonal de lapin anti-KATNB1

• Phare
• Validé par KD/KO

KATNB1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 14969-1-AP

Synonymes

p80 katanin, Katanin p80 WD40 repeat-containing subunit B1, Katanin p80 subunit B1, KAT

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Galerie de données de validation

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  WB analysis using 14969-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14969-1-AP (KATNB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14969-1-AP

  WB result of KATNB1 antibody (14969-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KATNB1 transfected HeLa cells.

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  WB analysis of mouse skeletal muscle using 14969-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14969-1-AP (KATNB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14969-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14969-1-AP (KATNB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 14969-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14969-1-AP (KATNB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14969-1-AP

  IP result of anti-KATNB1 (IP:14969-1-AP, 3ug; Detection:14969-1-AP 1:500) with HeLa cells lysate 3000ug.

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  IF Staining of HeLa using 14969-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14969-1-AP (KATNB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules HepG2, tissu de muscle squelettique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IF: See 7 publications below

Informations sur le produit

14969-1-AP cible KATNB1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KATNB1 Protéine recombinante Ag6836
• Nom complet: katanin p80 (WD repeat containing) subunit B 1
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 72-80 kDa
• Numéro d’acquisition GenBank: BC001353
• Symbole du gène: KATNB1
• Identification du gène (NCBI): 10300
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Katanin p80 WD40-containing subunit B1 is a protein that in humans is encoded by the KATNB1 gene. Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help to organize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60 subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemble microtubules, while the p80 subunit targets the enzyme to the centrosome. Katanin is a member of the AAA family of ATPases.

Publications

Species	Application	Title
mouse	WB, IF	Neuron; Katanin p80 regulates human cortical development by limiting centriole and cilia number.; Authors - Wen F Hu
human	WB	Dev Cell; Microtubule Minus-End Stabilization by Polymerization-Driven CAMSAP Deposition.; Authors - Kai Jiang
human	IF, WB	J Cell Biol; WDR62 localizes katanin at spindle poles to ensure synchronous chromosome segregation.; Authors - Amanda Guerreiro
human	WB, IF	J Cell Biol; WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin.; Authors - Junjie Huang
mouse	IF	J Neurosci; Functional inhibition of katanin affects synaptic plasticity; Authors - Franco L Lombino
human	WB	Structure; Structural Basis of Formation of the Microtubule Minus-End-Regulating CAMSAP-Katanin Complex.; Authors - Kai Jiang ; KO Validated

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