Anticorps Polyclonal de lapin anti-KCC4/SLC12A7
KCC4/SLC12A7 Polyclonal Antibody for WB, IF/ICC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain
Applications
WB, IF/ICC, ELISA
Conjugaison
Non conjugué
N° de cat : 26590-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules BGC-823, cellules NCI-H1299, cellules Raji, cellules SH-SY5Y |
| Résultats positifs en IF/ICC | cellules A431, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Informations sur le produit
26590-1-AP cible KCC4/SLC12A7 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | KCC4/SLC12A7 Protéine recombinante Ag24954 |
| Nom complet | solute carrier family 12 (potassium/chloride transporters), member 7 |
| Poids moléculaire observé | 119 kDa |
| Numéro d’acquisition GenBank | BC098390 |
| Symbole du gène | KCC4/SLC12A7 |
| Identification du gène (NCBI) | 10723 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
KCC4, also named as Solute carrier family 12 member 7(SLC12A7), is a 1083 amino-acid protein, which belongs to the SLC12A transporter family. As a multi-pass membrane protein, the molecular weight of KCC4 is 119 kDa. KCC4 mediates electroneutral potassium-chloride cotransport when activated by cell swelling. Moreover, KCC4 may mediate K+ uptake into Deiters' cells in the cochlea and contribute to K+ recycling in the inner ear. KCC4 is important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti(Uniprot, GeneID:10723).
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for KCC4/SLC12A7 antibody 26590-1-AP | Download protocol |
| IF protocol for KCC4/SLC12A7 antibody 26590-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Bishwa Ranjan (Verified Customer) (08-03-2023) | In the Western blots there is an additional band below at 98kD which seems like a degradation or non-specific binding of the antibody. The bands showing at 120-130kD are accurate as we have seen in the lanes from knockdowns and overexpression of protein KCC4 For reference information about the lanes: 1. MDA KCC4 overexpression 2. MDA KCC4 knockdown sequence1 3. MDA KCC4 knockdown sequence2 4. MDA KCC4 knockdown sequence3 5. MDA scrambled control As you can see in lane 5 which is the control has expression of kCC4 at 120-130 range and for the knockdowns there is a decrease more significantly for 2nd sequence and for overexpression in lane 1 it is quite high so we believe the top band is the expected one and the bottom one is a result of degradation maybe so please advice on this matter. As for the antibody we received it in April 2023 and it is stored at -20 as mentioned.
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