Anticorps Polyclonal de lapin anti-KCNIP3

KCNIP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 12032-1-AP

Synonymes

A-type potassium channel modulatory protein 3, Calsenilin, DRE-antagonist modulator, Kv channel-interacting protein 3

---

Galerie de données de validation

• 

  WB analysis of mouse brain using 12032-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12032-1-AP (KCNIP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12032-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12032-1-AP (KCNIP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12032-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12032-1-AP (KCNIP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12032-1-AP

  IP result of anti-KCNIP3 (IP:12032-1-AP, 4ug; Detection:12032-1-AP 1:1000) with mouse brain tissue lysate 4400 ug.

• 

  IHC staining of human gliomas using 12032-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12032-1-AP (KCNIP3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human gliomas using 12032-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12032-1-AP (KCNIP3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 12032-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using KCNIP3 antibody (12032-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

12032-1-AP cible KCNIP3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KCNIP3 Protéine recombinante Ag2665
• Nom complet: Kv channel interacting protein 3, calsenilin
• Masse moléculaire calculée: 256 aa, 29 kDa
• Poids moléculaire observé: 29 kDa, 27 kDa
• Numéro d’acquisition GenBank: BC012850
• Symbole du gène: KCNIP3
• Identification du gène (NCBI): 30818
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KCNIP3 also known as Calsenilin, CSEN, DREAM, or KCHIP3, is a protein-coding gene located on human chromosome 2q11.1 . It encodes a small calcium-binding protein belonging to the recoverin branch of the EF-hand superfamily. KCNIP3 is an integral subunit of native Kv4 channel complexes, regulating A-type potassium currents in response to intracellular calcium changes, thereby modulating neuronal excitability. KCNIP3 interacts with presenilin 1 (PSEN1) and presenilin 2 (PSEN2), influencing γ-secretase activity and β-amyloid formation, potentially linking it to Alzheimer's disease pathology.

Publications

Species	Application	Title
Human		Mol Med Rep; Protective effects of hydrogen sulfide against angiotensin II-induced endoplasmic reticulum stress in HUVECs.; Authors - Heng-Jing Hu

{{ptg:RelatedPrimaryAntibodies}}