Anticorps Polyclonal de lapin anti-Kir6.2

Kir6.2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 16920-1-AP

Synonymes

BIR, HHF2, IKATP, KCNJ11, KIR6.2, PHHI, TNDM3

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Galerie de données de validation

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  WB analysis of rat heart using 16920-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 16920-1-AP (Kir6.2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skeletal muscle using 16920-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16920-1-AP (Kir6.2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16920-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16920-1-AP (KCNJ11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16920-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16920-1-AP (KCNJ11 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de rat, cellules HepG2, tissu cardiaque humain, tissu de muscle squelettique de rat

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

16920-1-AP cible Kir6.2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Kir6.2 Protéine recombinante Ag10262
• Nom complet: potassium inwardly-rectifying channel, subfamily J, member 11
• Masse moléculaire calculée: 390 aa, 44 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC064497
• Symbole du gène: Kir6.2
• Identification du gène (NCBI): 3767
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Kir6.2 (also known as BIR or IKATP), encoded by the KCNJ11 gene, is the pore-forming unit of the ATP-sensitive K+ channel, an inward-rectifier potassium ion channel. Kir6.2 is controlled by G-proteins and is found associated with the sulfonylurea receptor (SUR) to constitute the ATP-sensitive K+ channel. The KCNJ11 gene is located at 11p15.1 and has no intron. Mutations in KCNJ11 are a cause of familial PHHI, an autosomal recessive disorder characterized by unregulated ins secretion. Defects in KCNJ11 may also contribute to autosomal dominant non-ins-dependent diabetes mellitus type II (NIDDM), transient neonatal diabetes mellitus type 3 (TNDM3), and permanent neonatal diabetes mellitus (PNDM).

Publications

Species	Application	Title
		Life Sci; KATP channels as ROS-dependent modulator of neurotransmitter release at the neuromuscular junctions; Authors - Andrei N Tsentsevitsky
rat	WB	Sci Rep; Glucocorticoid receptor-NECAB1 axis can negatively regulate insulin secretion in pancreatic β-cells; Authors - Haruhide Udagawa
mouse	WB	Int Immunopharmacol; Nicorandil restores endothelial cell Kir6.2 expression to alleviate neuropathic pain in mice after chronic constriction injury; Authors - Minghao Du

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