Anticorps Polyclonal de lapin anti-KCTD10

• Phare
• Validé par KD/KO

KCTD10 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (2)

N° de cat : 27279-1-AP

Synonymes

BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3, BTB/POZ domain-containing protein KCTD10, hBACURD3, MSTP028, Potassium channel tetramerization domain-containing protein 10

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Galerie de données de validation

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  WB analysis using 27279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27279-1-AP (KCTD10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HuH-7 using 27279-1-AP

  IP result of anti-KCTD10 (IP:27279-1-AP, 4ug; Detection:27279-1-AP 1:2000) with HuH-7 cells lysate 880 ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 27279-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27279-1-AP (KCTD10 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27279-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27279-1-AP (KCTD10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 27279-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27279-1-AP (KCTD10 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27279-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27279-1-AP (KCTD10 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate hyperplasia using 27279-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 27279-1-AP (KCTD10 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 27279-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 27279-1-AP (KCTD10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HuH-7, cellules Jurkat, cellules K-562, cellules MCF-7, cellules U2OS
• Résultats positifs en IP: cellules HuH-7,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu d'hyperplasie de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below

Informations sur le produit

27279-1-AP cible KCTD10 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KCTD10 Protéine recombinante Ag25971
• Nom complet: potassium channel tetramerisation domain containing 10
• Masse moléculaire calculée: 313 aa, 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC040062
• Symbole du gène: KCTD10
• Identification du gène (NCBI): 83892
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KCTD10 is a member of the polymerase delta-interacting protein 1 (PDIP1) gene family, which consists of 3 members, PDIP1, KCTD10 and TNFAIP1. KCTD10 is inducible by TNF-α, interacts with PCNA and the small subunit (p50) of DNA polymerase δ. Previous reports have shown that KCTD10 is essential for mammalian development. Further functional studies showed that KCTD10 interacts with proliferating cell nuclear antigen (PCNA) and plays an important role in DNA synthesis.

Publications

Species	Application	Title
human	WB	Mol Cell; Unbiased interrogation of functional lysine residues in human proteome; Authors - Ying Bao
human	WB	Nat Commun; Regulation of TRIF-mediated innate immune response by K27-linked polyubiquitination and deubiquitination.; Authors - Xin Wu ; KO Validated
mouse	WB	Front Cell Dev Biol; Cullin3-TNFAIP1 E3 Ligase Controls Inflammatory Response in Hepatocellular Carcinoma Cells via Ubiquitination of RhoB.; Authors - Yue Liu ; KD Validated
rat,human	WB	Genet Res (Camb); Inhibition of KCTD10 Affects Diabetic Retinopathy Progression by Reducing VEGF and Affecting Angiogenesis; Authors - Yun Feng ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vasu (Verified Customer) (09-12-2025)

This antibody is suitable for few applications like immunofluroscence and Immunoblotting but not for other applications like Chip and flow cytometry and other applications

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Protein purification
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Breast and Cervical Cancer cells

FH

Vasudevarao (Verified Customer) (09-04-2025)

This antibody works very well for immunoblotting and IHC at the recommended dilutions

• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Prostate and Breast Cancer cells

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