Anticorps Polyclonal de lapin anti-KDM2A

KDM2A Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 24311-1-AP

Synonymes

CXXC8, F box protein FBL7, F box protein Lilina, F box/LRR repeat protein 11, FBL11, FBL7, FBXL11, JHDM1A, KDM2A, KIAA1004, LILINA, lysine (K) demethylase 2A, Lysine specific demethylase 2A

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Galerie de données de validation

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  WB analysis of Jurkat using 24311-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24311-1-AP (KDM2A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 24311-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24311-1-AP (KDM2A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24311-1-AP

  IP Result of anti-KDM2A (IP:24311-1-AP, 4ug; Detection:24311-1-AP 1:600) with HeLa cells lysate 1600ug.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

24311-1-AP cible KDM2A dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KDM2A Protéine recombinante Ag19448
• Nom complet: lysine (K)-specific demethylase 2A
• Masse moléculaire calculée: 1162 aa, 133 kDa
• Poids moléculaire observé: 126 kDa
• Numéro d’acquisition GenBank: BC064360
• Symbole du gène: KDM2A
• Identification du gène (NCBI): 22992
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KDM2A(Lysine-specific demethylase 2A) is also named as CXXC8, FBL7, FBXL11, JHDM1A, KIAA1004 and belongs to the JHDM1 histone demethylase family. It is required to maintain the heterochromatic state, and playing a role in epigenetic silencing. KDM2A is one of the four subunits of the E3 ubiquitin protein ligase complex (SCF) with SKP1, CUL1, RBX1, Fbox protein bringing ubiquitin conjugating enzymes to substrates recruited by F-box.

Publications

Species	Application	Title
human	WB,IF,CoIP	Cell Oncol (Dordr); Hepatitis B virus core protein stabilizes RANGAP1 to upregulate KDM2A and facilitate hepatocarcinogenesis; Authors - Hong-Juan You
human	WB	J Cell Mol Med; Circular RNA circFOXO3 regulates KDM2A by targeting miR-214 to promote tumor growth and metastasis in oral squamous cell carcinoma.; Authors - Yilong Ai
human	WB	J Cell Physiol; The Ubiquitin Ligase SCF(FBXW7α) Promotes ATA3 Degradation.; Authors - Nan Song
human	IP, WB	Cell Rep; Enhancer RNAs Mediate Estrogen-Induced Decommissioning of Selective Enhancers by Recruiting ERα and Its Cofactor.; Authors - Mei Yang
human	WB,IHC	Cell Death Dis; CircRNF144B/miR-342-3p/FBXL11 axis reduced autophagy and promoted the progression of ovarian cancer by increasing the ubiquitination of Beclin-1; Authors - Wenting Song