Anticorps Polyclonal de lapin anti-RBP2

• Phare
• Validé par KD/KO

RBP2 Polyclonal Antibody for WB, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 18825-1-AP

Synonymes

KDM5A, [histone H3]-trimethyl-L-lysine(4) demethylase 5A, EC:1.14.11.67, JARID1A, Jumonji/ARID domain-containing protein 1A

---

Galerie de données de validation

• 

  WB analysis of HeLa using 18825-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18825-1-AP (RBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 18825-1-AP

  WB result of KDM5A antibody (18825-1-AP; 1:600; room temperature for 1.5 hours) with wild-type and KDM5A knockout HEK-293T cells.

• 

  IP experiment of HeLa using 18825-1-AP

  IP result of anti-RBP2 (IP:18825-1-AP, 4ug; Detection:18825-1-AP 1:500) with HeLa cells lysate 1280 ug.

• 

  IF Staining of mouse small intestine using 18825-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using RBP2 antibody (18825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse small intestine using 18825-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using RBP2 antibody (18825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18825-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18825-1-AP (RBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293T
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IF-P: tissu d'intestin grêle de souris,
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

18825-1-AP cible RBP2 dans les applications de WB, IF/ICC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: lysine (K)-specific demethylase 5A
• Masse moléculaire calculée: 192 kDa
• Poids moléculaire observé: 192 kDa
• Numéro d’acquisition GenBank: NM_005056
• Symbole du gène: KDM5A
• Identification du gène (NCBI): 5927
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KDM5A, also named as JARID1A, RBBP2 and RBP2, belongs to the JARID1 histone demethylase family. KDM5A is a Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. RBP2 may stimulate transcription mediated by nuclear receptors. It may be involved in transcriptional regulation of Hox proteins during cell differentiation. It may participate in transcriptional repression of cytokines such as CXCL12.

Publications

Species	Application	Title
mouse	WB	iScience; Matrix stiffness aggravates osteoarthritis progression through H3K27me3 demethylation induced by mitochondrial damage; Authors - Tianyou Kan
mouse	WB,IF	Environ Toxicol; JMJD3 downregulates IL4i1 aggravating lipopolysaccharide-induced acute lung injury via H3K27 and H3K4 demethylation; Authors - Yizhuo Gao
human	WB	Int J Biol Sci; KDM5B demethylates H3K4 to recruit XRCC1 and promote chemoresistance.; Authors - Wenxia Xu
rat	IF	Life Sci; Treadmill exercise improves neurological function by inhibiting autophagy and the binding of HMGB1 to Beclin1 in MCAO juvenile rats.; Authors - Guoyuan Pan

{{ptg:RelatedPrimaryAntibodies}}