Anticorps Polyclonal de lapin anti-KHDRBS3

• Phare
• Validé par KD/KO

KHDRBS3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (3)

N° de cat : 13563-1-AP

Synonymes

Etle, etoile, KHDRBS3, RNA binding protein T Star, SALP, Sam68 like mammalian protein 2, SLM 2, SLM2, T STAR, TSTAR

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Galerie de données de validation

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  WB analysis of mouse brain using 13563-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13563-1-AP (KHDRBS3 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BGC-823 using 13563-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 13563-1-AP (KHDRBS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13563-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13563-1-AP (KHDRBS3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13563-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13563-1-AP (KHDRBS3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13563-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13563-1-AP (KHDRBS3 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 13563-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13563-1-AP (KHDRBS3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human gliomas using 13563-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13563-1-AP (KHDRBS3 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549, cellules BGC-823, tissu cérébral humain
• Résultats positifs en IHC: tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13563-1-AP cible KHDRBS3 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KHDRBS3 Protéine recombinante Ag4438
• Nom complet: KH domain containing, RNA binding, signal transduction associated 3
• Masse moléculaire calculée: 346 aa, 38, 8 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC032606
• Symbole du gène: KHDRBS3
• Identification du gène (NCBI): 10656
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KH domain-containing, RNA-binding, signal transduction-associated protein 3 (KHDRBS3, SLM2) is a RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion (15901763). May play a role as a negative regulator of cell growth. Inhibits cell proliferation. Involved in splice site selection of vascular endothelial growth factor. Induces an increased concentration-dependent incorporation of exon in CD44 pre-mRNA by direct binding to purine-rich exonic enhancer. RNA-binding abilities are down-regulated by tyrosine kinase PTK6. Involved in post-transcriptional regulation of HIV-1 gene expression (11741900).Ubiquitous with higher expression in testis, skeletal muscle and brain. Expressed in the kidney only in podocytes, the glomerular epithelial cells of the kidney. Strongly expressed after meiosis.This antibody is a rabbit polyclonal antibody raised against a full-length human KHDRBS3 protein.The observed molecular weight of KHDRBS is about 55kd.

Publications

Species	Application	Title
human	IHC	Acta Neuropathol; Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.; Authors - Neumann Manuela M
mouse	WB	PLoS Genet; The tissue-specific RNA binding protein T-STAR controls regional splicing patterns of neurexin pre-mRNAs in the brain.; Authors - Ehrmann Ingrid I
human	WB	Am J Reprod Immunol; Long non-coding RNA TPT1-AS1 inhibits ferroptosis in ovarian cancer by regulating GPX4 via CREB1 regulation; Authors - Lei Cao
human	WB	Cell Death Dis; KHDRBS3 regulates the permeability of blood-tumor barrier via cDENND4C/miR-577 axis.; Authors - Peiqi Wu ; KD Validated
human	WB	Life Sci; KHDRBS3 facilitates self-renewal and temozolomide resistance of glioblastoma cell lines; Authors - Kanokkuan Somrit
human	WB,IF,CoIP	Int J Nanomedicine; Exosome-Delivered circFOXP1 Upregulates Autophagy and Promotes Hepatocellular Carcinoma Progression Through Its Encoded p196 Protein Targeting the KHDRBS3/ULK1 Axis; Authors - Peng Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (11-10-2024)

Antibodies were used to detect Khdrbs3 in human RKO cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RKO

KHDRBS3 Antibody Western Blot validation (1:1000 dilution) in RKO (Cat no:13563-1-AP)

FH

Tsimafei (Verified Customer) (08-07-2024)

Great antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mESCs

KHDRBS3 Antibody Western Blot validation (1:2000 dilution) in mESCs (Cat no:13563-1-AP)

FH

Julia (Verified Customer) (08-07-2024)

Antibody used for 30ug of mESC protein extract. There are few bands and strong signal at ~55kDa so it can be diluted even more than 1:10000 for similar set-up.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: mESC

KHDRBS3 Antibody Western Blot validation (1:10000 dilution) in mESC (Cat no:13563-1-AP)

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