Anticorps Polyclonal de lapin anti-KHK

• Phare
• Validé par KD/KO

KHK Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 15681-1-AP

Synonymes

Hepatic fructokinase, Ketohexokinase, ketohexokinase (fructokinase), KHK

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Galerie de données de validation

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  WB analysis using 15681-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15681-1-AP (KHK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15681-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15681-1-AP (KHK antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15681-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15681-1-AP (KHK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15681-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15681-1-AP (KHK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15681-1-AP

  IP Result of anti-KHK (IP:15681-1-AP, 4ug; Detection:15681-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IP experiment of HepG2 using 15681-1-AP

  IP result of anti-KHK(IP:15681-1-AP, 4ug; Detection:15681-1-AP 1:1000) with HepG2 cells lysate 960 ug.

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  IHC staining of human liver using 15681-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15681-1-AP (KHK Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 15681-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15681-1-AP (KHK Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human liver using 15681-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15681-1-AP (KHK Antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human liver using 15681-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15681-1-AP (KHK Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 15681-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 15681-1-AP (KHK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15681-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using KHK antibody (15681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de rat, tissu hépatique humain
• Résultats positifs en IP: tissu hépatique de souris, cellules HepG2
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below

Informations sur le produit

15681-1-AP cible KHK dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KHK Protéine recombinante Ag8182
• Nom complet: ketohexokinase (fructokinase)
• Masse moléculaire calculée: 298 aa, 33 kDa
• Poids moléculaire observé: 33-35 kDa
• Numéro d’acquisition GenBank: BC006233
• Symbole du gène: KHK
• Identification du gène (NCBI): 3795
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ketohexokinase catalyzes conversion of fructose to fructose-1-phosphate, it is the first enzyme with a specialized pathway that catabolizes dietary fructose. Fructose metabolism seems to provide cancer cells with the supplementary fuel required for proliferation and metastasis.

Publications

Species	Application	Title
mouse	WB,IHC	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
human	WB	Adv Sci (Weinh); NAT10/ac4C/FOXP1 Promotes Malignant Progression and Facilitates Immunosuppression by Reprogramming Glycolytic Metabolism in Cervical Cancer; Authors - Xiaona Chen
human	WB	Brain; C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.; Authors - Scott P Allen
mouse	WB	Endocrinology; Hepatic but not intestinal FBP1 is required for fructose metabolism and tolerance; Authors - Qiufang Bai
human	WB	Biochem Biophys Res Commun; Ketohexokinase is involved in fructose utilization and promotes tumor progression in glioma.; Authors - Wenbo Gao
human	WB	PeerJ; Increased utilization of fructose has a positive effect on the development of breast cancer.; Authors - Xiajing Fan ; KD Validated