Anticorps Polyclonal de lapin anti-KIAA1033

KIAA1033 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (1)

N° de cat : 51101-1-AP

Synonymes

Strumpellin and WASH-interacting protein, SWIP, WASH complex subunit 4, WASH complex subunit 7, WASH complex subunit SWIP

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Galerie de données de validation

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  WB analysis using 51101-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51101-1-AP (KIAA1033 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 51101-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51101-1-AP (KIAA1033 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 51101-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 51101-1-AP (KIAA1033 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 51101-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 51101-1-AP (KIAA1033 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

51101-1-AP cible KIAA1033 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: KIAA1033
• Masse moléculaire calculée: 136.4 kDa
• Poids moléculaire observé: 136 kDa
• Numéro d’acquisition GenBank: BC104994
• Symbole du gène: KIAA1033
• Identification du gène (NCBI): 23325
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KIAA1033, also named as WASH-interacting protein and SWIP, is a component of WASH complex. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting. KIAA1033 has two isoforms with MW 100 kDa and 136 kDa.

Publications

Species	Application	Title
Human	WB	Mol Cell Proteomics; Metabolic Enzyme Alterations and Astrocyte Dysfunction in a Murine Model of Alexander Disease With Severe Reactive Gliosis.; Authors - Michael R Heaven
		Sci Rep; Starvation-induced metabolic rewiring affects mTORC1 composition in vivo; Authors - Kaade Edgar
mouse	WB	Pigment Cell Melanoma Res; Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour.; Authors - Benjamin J Tyrrell
mouse	WB	Immunology; SNX17 Regulates Antigen Internalisation and Phagosomal Maturation by Dendritic Cells; Authors - Sofía Dinamarca
human	WB	Autophagy; RAB21 controls autophagy and cellular energy homeostasis by regulating retromer-mediated recycling of SLC2A1/GLUT1; Authors - Yifei Pei

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ioana (Verified Customer) (08-28-2025)

It worked well for Western Blotting, band size as expected and good intensity.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLaM

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