Anticorps Polyclonal de lapin anti-VIRMA/KIAA1429

• Phare
• Validé par KD/KO

VIRMA/KIAA1429 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(28)

N° de cat : 25712-1-AP

Synonymes

KIAA1429, VIRMA, MSTP054, virilizer

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Galerie de données de validation

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  WB analysis using 25712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 25712-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25712-1-AP (KIAA1429 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 25712-1-AP

  IP result of anti-VIRMA/KIAA1429 (IP:25712-1-AP, 4ug; Detection:25712-1-AP 1:600) with MCF-7 cells lysate 800 ug.

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  IHC staining of human breast cancer using 25712-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 25712-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 25712-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 25712-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 25712-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules MDA-MB-231, cellules PC-3, cellules SKOV-3, cellules SW480
• Résultats positifs en IP: cellules MCF-7,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du foie humain, tissu testiculaire de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 19 publications below
• IHC: See 9 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

25712-1-AP cible VIRMA/KIAA1429 dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VIRMA/KIAA1429 Protéine recombinante Ag22449
• Nom complet: KIAA1429
• Masse moléculaire calculée: 1812 aa, 202 kDa
• Poids moléculaire observé: 200-210 kDa
• Numéro d’acquisition GenBank: BC113380
• Symbole du gène: KIAA1429
• Identification du gène (NCBI): 25962
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as an oncogenic factor in breast cancer and liver cancer. KIAA1429 exhibits differential nuclear and cytoplasmic distribution in colorectal cancer samples, and high cytoplasmic expression of KIAA1429 is associated with poor response to chemotherapy. BRAF-mediated phosphorylation of KIAA1429 facilitates its aggregation in the cytoplasm. Furthermore, cytoplasmic KIAA1429 promotes WNT pathway activation by binding and stabilizing FZD7, thereby further enhancing cancer stemness and oxaliplatin resistance (PMID: 40611274).

Publications

Species	Application	Title
human	WB	Dev Cell; PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.; Authors - Deguan Lv
mouse,human	WB,IP,IHC	Cell Mol Biol Lett; KIAA1429-mediated m6A modification of CHST11 promotes progression of diffuse large B-cell lymphoma by regulating Hippo-YAP pathway; Authors - Xiaomin Chen ; KO Validated
human	IF	Cell Rep; Pathological α-synuclein dysregulates epitranscriptomic writer METTL3 to drive neuroinflammation in microglia; Authors - Cameron Miller
human	WB	Aging (Albany NY); WTAP promotes myocardial ischemia/reperfusion injury by increasing endoplasmic reticulum stress via regulating m6A modification of ATF4 mRNA.; Authors - Jiayi Wang
human	WB	Front Oncol; m6A Methyltransferase METTL14-Mediated Upregulation of Cytidine Deaminase Promoting Gemcitabine Resistance in Pancreatic Cancer.; Authors - Congjun Zhang
human	WB,CoIP	Sci Rep; The m6A-methylase complex recruits TREX and regulates mRNA export.; Authors - Simon Lesbirel ; KD Validated

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