Anticorps Monoclonal anti-CIP2A

CIP2A Monoclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IF,ELISA

Conjugaison

Non conjugué

CloneNo.

2A3F3

N° de cat : 67843-1-Ig

Synonymes

Cancerous inhibitor of PP2A, CIP2A, FLJ12850, KIAA1524, p90, p90 autoantigen, Protein CIP2A

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Galerie de données de validation

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  WB analysis using 67843-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67843-1-Ig (KIAA1524 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67843-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67843-1-Ig (KIAA1524 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 67843-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67843-1-Ig (KIAA1524 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 67843-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KIAA1524 antibody (67843-1-Ig, Clone: 2A3F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules PC-12
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67843-1-Ig cible CIP2A dans les applications de WB, IF,ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CIP2A Protéine recombinante Ag19598
• Nom complet: KIAA1524
• Masse moléculaire calculée: 905 aa, 102 kDa
• Poids moléculaire observé: 102 kDa
• Numéro d’acquisition GenBank: BC130564
• Symbole du gène: KIAA1524
• Identification du gène (NCBI): 57650
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cancerous inhibitor of protein phosphatase 2A (CIP2A) was originally identified as a cellular protein phosphatase 2A (PP2A) inhibitor, and has been shown to control oncogenic cellular signals by suppressing the tumor suppressor PP2A. CIP2A overexpression has been found in several human malignancies including breast cancer, hepatocellular carcinoma, gastric cancer, head and neck cancer, colon cancer, prostate cancer and non-small cell lung cancer. CIP2A is a 83~102 kDa cytoplasmic protein. ( PMID: 26824320, PMID: 25458953, PMID: 29720672)