Anticorps Polyclonal de lapin anti-Eg5

• Phare
• Validé par KD/KO

Eg5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (1)

N° de cat : 23333-1-AP

Synonymes

EG5, HKSP, KIF11, kinesin family member 11, Kinesin like protein 1, Kinesin like protein KIF11, KNSL1, TR interacting protein 5, TRIP 5, TRIP5

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Galerie de données de validation

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  WB analysis using 23333-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23333-1-AP (Eg5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 23333-1-AP

  IP Result of anti-Eg5 (IP:23333-1-AP, 4ug; Detection:23333-1-AP 1:1000) with Raji cells lysate 2000ug.

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  IHC staining of human lung cancer using 23333-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 23333-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 23333-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 23333-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 23333-1-AP (Eg5 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 23333-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 23333-1-AP (Eg5 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human endometrial cancer using 23333-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 23333-1-AP (Eg5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 23333-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Eg5 antibody (23333-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 23333-1-AP

  Immunofluorescent analysis of HepG2 cells using 23333-1-AP (Eg5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, cellules Raji
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de l'endomètre humain, tissu d'hyperplasie de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 7 publications below
• IF: See 2 publications below

Informations sur le produit

23333-1-AP cible Eg5 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Eg5 Protéine recombinante Ag18695
• Nom complet: kinesin family member 11
• Masse moléculaire calculée: 1056 aa, 119 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: BC136474
• Symbole du gène: KIF11
• Identification du gène (NCBI): 3832
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.

Publications

Species	Application	Title
mouse	IHC	Int J Oncol; Cyclovirobuxine D inhibits growth and progression of non‑small cell lung cancer cells by suppressing the KIF11‑CDC25C‑CDK1‑CyclinB1 G2/M phase transition regulatory network and the NFκB/JNK signaling pathway; Authors - Ting Xue
human	IHC	Int J Cancer; Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.; Authors - Fangqiu Fu
human	WB	Int J Biol Sci; KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer.; Authors - Dang Wei
human	WB	Mol Carcinog; Kinesin family member 11 is a potential therapeutic target and is suppressed by microRNA-30a in breast cancer.; Authors - Benfang Wang ; KD Validated
human	WB	Am J Transl Res; Kinesin family members KIF2C/4A/10/11/14/18B/20A/23 predict poor prognosis and promote cell proliferation in hepatocellular carcinoma.; Authors - Xishan Li
human	WB,IHC	Neurooncol Adv; KIF11 and KIF15 mitotic kinesins are potential therapeutic vulnerabilities for malignant peripheral nerve sheath tumors.; Authors - Ernest Terribas

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iram (Verified Customer) (01-15-2021)

Eg5 antibody giving a very clean bands and good staining

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC and IF
• Cell Tissue Type: Human colon cancer cells and tissue