Anticorps Polyclonal de lapin anti-KIF12

KIF12 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (1)

N° de cat : 12035-1-AP

Synonymes

Kinesin like protein KIF12, Kinesin-like protein KIF12, RP11 56P10.3

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Galerie de données de validation

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  WB analysis using 12035-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12035-1-AP (KIF12 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12035-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12035-1-AP (KIF12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 12035-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12035-1-AP (KIF12 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 12035-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12035-1-AP (KIF12 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 12035-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12035-1-AP (KIF12 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 12035-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12035-1-AP (KIF12 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 12035-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12035-1-AP (KIF12 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 12035-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12035-1-AP (KIF12 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 12035-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12035-1-AP (KIF12 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules Y79, tissu cardiaque de souris, tissu pancréatique de souris, tissu rénal de rat
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du pancréas humain, tissu de carcinome à cellules rénales humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

12035-1-AP cible KIF12 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIF12 Protéine recombinante Ag2707
• Nom complet: kinesin family member 12
• Masse moléculaire calculée: 646 aa, 71 kDa
• Poids moléculaire observé: 55-70 kDa
• Numéro d’acquisition GenBank: BC010626
• Symbole du gène: KIF12
• Identification du gène (NCBI): 113220
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IHC	J Am Soc Nephrol; HNF-1beta regulates transcription of the PKD modifier gene Kif12.; Authors - Gong Yimei Y
mouse,human	IF	Kidney Int; Copy number variation analysis identifies novel CAKUT candidate genes in children with a solitary functioning kidney.; Authors - Rik Westland
human	WB	Breast Cancer Res Treat; Specific kinesin expression profiles associated with taxane resistance in basal-like breast cancer.; Authors - Tan Min Han MH
human	IHC	J Proteomics; Proteomic analysis of podocyte exosome-enriched fraction from normal human urine.; Authors - Prunotto Marco M
human	WB,IF	J Pediatr; KIF12 Variants and Disturbed Hepatocyte Polarity in Children With a Phenotypic Spectrum of Cholestatic Liver Disease.; Authors - Stalke Amelie

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Huxley (Verified Customer) (07-28-2025)

Did not work for western blotting on cell lysates. Bands on western blot were the same in KIF12 knock-out cells (the knockout of KIF12 had been confirmed by genotyping) and in cells overexpressing KIF12-GFP as in control cells, so they must be nonspecific bands. However, ProteinTech's customer service was excellent and they promptly issued a refund for this.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Kidney epithelial cells

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