Anticorps Polyclonal de lapin anti-KIF1B

• Phare
• Validé par KD/KO

KIF1B Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 15263-1-AP

Synonymes

CMT2, CMT2A, CMT2A1, HMSNII, KIAA0591, KIAA1448, KIF1B, kinesin family member 1B, Kinesin like protein KIF1B, KLP

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Galerie de données de validation

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  WB analysis of HeLa using 15263-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15263-1-AP (KIF1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15263-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15263-1-AP (KIF1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15263-1-AP

  IP Result of anti-KIF1B (IP:15263-1-AP, 5ug; Detection:15263-1-AP 1:700) with HeLa cells lysate 2000ug.

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  IHC staining of human liver cancer using 15263-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15263-1-AP (KIF1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 15263-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15263-1-AP (KIF1B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 15263-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15263-1-AP (KIF1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cérébral de souris, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

15263-1-AP cible KIF1B dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIF1B Protéine recombinante Ag7263
• Nom complet: kinesin family member 1B
• Masse moléculaire calculée: 204 kDa
• Poids moléculaire observé: 199 kDa, 204 kDa
• Numéro d’acquisition GenBank: BC001415
• Symbole du gène: KIF1B
• Identification du gène (NCBI): 23095
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	IP	J Hazard Mater; Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria.; Authors - Yiyuan Kang
mouse	IF	Elife; Brucella activates the host RIDD pathway to subvert BLOS1-directed immune defense.; Authors - Kelsey Michelle Wells
mouse	WB	Exp Neurol; Axonal injury mediated by neuronal p75NTR/TRAF6/JNK pathway contributes to cognitive impairment after repetitive mTBI; Authors - Gang Liu
human	WB	Cell Rep; Microtubule-Driven Stress Granule Dynamics Regulate Inhibitory Immune Checkpoint Expression in T Cells.; Authors - Don-Marc Franchini ; KD Validated
rat	WB	Cell Death Dis; AAV-mediated inhibition of ULK1 promotes axonal regeneration in the central nervous system in vitro and in vivo.; Authors - Vinicius Toledo Ribas

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Maria (Verified Customer) (07-31-2021)

KIF1B in human primary fibroblasts in culture. Reducing and denaturing conditions. 15 ug of total protein. TGX gel 4-15%. Blocking BSA 3%. 1:1000 primary ab O/N 4ºC. 1:5000 secondary HRP antibody 2h RT. Many bands in the blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human primary fibroblasts

KIF1B Antibody Western Blot validation (1:1000 dilution) in Human primary fibroblasts (Cat no:15263-1-AP)