Anticorps Monoclonal anti-KIF20A

• Phare
• Validé par KD/KO

KIF20A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(11)

CloneNo.

2A8E3

Avis (1)

N° de cat : 67190-1-Ig

Synonymes

2A8E3, FLJ21151, GG10_2, Kinesin-like protein KIF20A, Mitotic kinesin-like protein 2

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Galerie de données de validation

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  WB analysis using 67190-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67190-1-Ig (KIF20A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of HeLa using 67190-1-Ig

  WB result of KIF20A antibody (67190-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF20A transfected HeLa cells.

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  WB analysis using 67190-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67190-1-Ig (KIF20A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 67190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67190-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KIF20A antibody (67190-1-Ig, Clone: 2A8E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules HeLa, cellules HL-60, cellules HSC-T6, cellules Jurkat, cellules MDA-MB-468, cellules NIH/3T3, cellules PC-12
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Informations sur le produit

67190-1-Ig cible KIF20A dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: KIF20A Protéine recombinante Ag8916
• Nom complet: kinesin family member 20A
• Masse moléculaire calculée: 890 aa, 100 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC012999
• Symbole du gène: KIF20A
• Identification du gène (NCBI): 10112
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KIF20A, also known as RAB6KIFL or MKlp2, is a kinesin originally identified as a binding partner for the rab6 GTPase involved in protein transport at the Golgi apparatus. KIF20A belongs to a large family of motor proteins that accumulate in mitotic cells and is required for normal cell division. KIF20A is thought to be involved in carcinogenesis. In normal tissues KIF20A is almost undetectable while it is overexpressed in various cancers. KIF20A has been identified as a novel promising candidate for anticancer immunotherapy.

Publications

Species	Application	Title
mouse	WB	Dev Cell; DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice; Authors - Dingfeng Zou
human	IF	Proc Natl Acad Sci U S A; Maternal genetic variants in kinesin motor domains prematurely increase egg aneuploidy; Authors - Leelabati Biswas
human	IF	Cancer Res; Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.; Authors - Bingteng Xie
mouse,human	WB,IF	Genomics; Upregulation of the key biomarker kinesin family member 20A (KIF20A) is associated with the pulmonary artery hypertension; Authors - Linqing Li
mouse	IF	medRxiv; Maternal genetic variants in kinesin motor domains prematurely increase egg aneuploidy; Authors - Leelabati Biswas
mouse	IF	Res Sq; An oocyte meiotic midbody cap is required for developmental competence in mice; Authors - Karen Schindler

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wissem (Verified Customer) (07-30-2024)

antibody works well to detect KIF20A in U2OS cell extracts

• Applications: Western Blot
• Primary Antibody Dilution: 1:10,000

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