Anticorps Polyclonal de lapin anti-KIF22

• Phare
• Validé par KD/KO

KIF22 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 13403-1-AP

Synonymes

KID, KIF22, kinesin family member 22, Kinesin like protein 4, Kinesin like protein KIF22, KNSL4, OBP, OBP 1, OBP 2

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Galerie de données de validation

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  WB analysis using 13403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13403-1-AP (KIF22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13403-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13403-1-AP (KIF22 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13403-1-AP

  IP Result of anti-KIF22 (IP:13403-1-AP, 4ug; Detection:13403-1-AP 1:300) with HeLa cells lysate 4000ug.

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  IHC staining of human cervical cancer using 13403-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13403-1-AP (KIF22 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 13403-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13403-1-AP (KIF22 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 13403-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13403-1-AP (KIF22 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13403-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13403-1-AP (KIF22 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules MKN-45, cellules T-47D, tissu cardiaque humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

13403-1-AP cible KIF22 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIF22 Protéine recombinante Ag4254
• Nom complet: kinesin family member 22
• Masse moléculaire calculée: 665 aa, 73 kDa
• Poids moléculaire observé: 68-73 kDa
• Numéro d’acquisition GenBank: BC028155
• Symbole du gène: KIF22
• Identification du gène (NCBI): 3835
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KIF22, also known as KID (kinesin-like DNA-binding protein), is a kinesin-like DNA-binding protein known to be involved in chromosome movement during mitosis. KIF22 is a nuclear protein regulated by SIAH-1, whose level fluctuate in a cell cycle-dependent manner, increasing during pre-mitotic phases and greatly decreasing during mitosis. KIF22 localizes to the nucleus of the interphase cells upon entry into mitosis and displays some punctate cytoplasmic staining. Defects in KIF22 are the cause of spondyloepimetaphyseal dysplasia with joint laxity, type 2 (SEMDJL2).

Publications

Species	Application	Title
mouse	IF	Sci Rep; PMID: 29229923; Authors - Sara Bizzotto
human	WB	Proteomics; Genistein-induced mitotic arrest of gastric cancer cells by downregulating KIF20A, a proteomics study.; Authors - Yan Guang-Rong GR
human	WB,IHC	Chin Med J (Engl); Effect of KIF22 on promoting proliferation and migration of gastric cancer cells via MAPK-ERK pathways.; Authors - Ze-Yuan Yu ; KD Validated