Anticorps Polyclonal de lapin anti-KIF26B

• Phare
• Validé par KD/KO

KIF26B Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 17422-1-AP

Synonymes

KIF26B, kinesin family member 26B, Kinesin like protein KIF26B

---

Galerie de données de validation

• 

  WB analysis of MDCK using 17422-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 17422-1-AP (KIF26B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17422-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17422-1-AP (KIF26B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17422-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17422-1-AP (KIF26B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17422-1-AP (KIF26B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17422-1-AP (KIF26B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17422-1-AP (KIF26B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17422-1-AP (KIF26B Antibody) at dilution of 1:200 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: cellules MDCK, cellules HEK-293, cellules MCF-7
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 8 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Informations sur le produit

17422-1-AP cible KIF26B dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons canin, Humain

• Réactivité: canin, Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIF26B Protéine recombinante Ag11446
• Nom complet: kinesin family member 26B
• Masse moléculaire calculée: 2108aa,224 kDa; 162aa,17 kDa
• Poids moléculaire observé: 224-230 kDa, 17-22 kDa
• Numéro d’acquisition GenBank: BC042481
• Symbole du gène: KIF26B
• Identification du gène (NCBI): 55083
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KIF26B is a member of kinesin-11 family and plays important role in embryogenesis. It is a downstram target of Sall1 and is essential for kidney development. KIF26B is also involved in tumorigenesis.

Publications

Species	Application	Title
human,mouse	WB,IHC	Nat Commun; Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.; Authors - Shujie Chen ; KD Validated
human	IHC,WB	Biomed Pharmacother; KIF26B promotes cell proliferation and migration through the FGF2/ERK signaling pathway in breast cancer.; Authors - Yan Teng
human,mouse	WB,IF	J Bone Miner Res; KIF26B Silencing Prevents Osseous Transdifferentiation of Progenitor/Stem Cells and Attenuates Ectopic Calcification in a Murine Model.; Authors - Mingming Yan ; KD Validated
human	IF	J Ginseng Res; Ginsenoside Rh2 reduces m6A RNA methylation in cancer via the KIF26B-SRF positive feedback loop; Authors - Chunmei Hu
human	IHC	Cell Cycle; Noncoding RNAs-based high KIF26B expression correlates with poor prognosis and tumor immune infiltration in colon cancer; Authors - Zhihong Liu
mouse	IF	Biochem Biophys Res Commun; Novel mechanistic role of Kif26b in adipogenic differentiation of murine multipotent stromal cells.; Authors - Mingming Yan ; KD Validated